Ca2+调控骨髓基质干细胞BMP-2、Ang-1成骨及血管化的作用机制研究

Calcium ions play a key role in the early osteogenic process of bone marrow stem cells in bone graft material, but rhe mechanism of Ca2+ to regulate osteogenesis and vascularization of BMSCs is not clear. Our previous study showed that Ca2+ can affect the viability and differentiation of BMSCs in vitro, and vascularization was accompanied with osteogenesis in early process in vivo. So we hypothesized that Ca2+ affect the expression of BMP-2, Ang-1 and other proteins, and was regulated though p13K/Akt Signaling pathways. We will use Receptor blockade, carrier signal channel inhibition and transgenic technology to study the osteogenesis, osteogenic protein expression and signaling pathways in vitro and vivo, explore Ca2+ regulate the osteogenesis and vascularization of BMP-2 and Ang1 through p13K/Akt Signaling pathways. This study can illuminate that Ca2+ Play an important role in the osteogenesis and vascularization, and can lay the foundation to explain the mechanism of osteogenesis in bone formed of inorganic materials.

钙离子在骨材料早期骨髓基质干细胞成骨过程中起关键作用，但钙离子对于骨髓基质干细胞成骨分化及血管化中的作用机制不清，我们前期工作发现钙离子对于骨髓基质干细胞增殖及分化起重要作用，动物模型表明骨髓基质干细胞成骨早期伴随血管化增生明显，据此提出假设钙离子作用于骨髓基质干细胞后BMP-2、Ang-1表达及成骨相关指标增加，受p13K/Akt通路共同调控，我们将用受体阻滞、信号通道抑制及转基因载体技术进行细胞及动物模型分析成骨及蛋白表达、信号通道研究，探索钙离子通过p13K/Akt通路调控BMP-2、Ang-1成骨及血管化，对阐明钙离子在成骨及成血管化中的作用机制有重要意义，为解释生物无机材料成骨作用机理奠定基础。