类核-壳构型超亲水钙钛矿量子点合成及单细胞多元成像应用

The analysis of single cell is an important way to study the micro environment of cells, which can reflect the individual difference of cells caused by random biochemical reaction, and get more accurate and comprehensive information of the physiological state and process of cells. All inorganic perovskite nanocrystals (PNCs) have become the focus of research in many fields because of their excellent optical properties. In this proposal, we plan to start from the preparation of super-hydrophilic PNCs, focus on the basic issues of synthesis of multiband PNCs, surface functionalized modification and cell imaging to explore new method for multiple imaging analysis in a single cell based on the core-shell-like structure and hydrophilic multiband PNCs. The optical properties, affinity/hydrophobicity and biocompatibility of the multiband PNCs probes in water were studied by controlling the core elements, reaction conditions and the types of (charged) hydrophilic/hydrophobic polymers. Combined with bioimaging technology, the multiple imaging analysis in single cell will be achieved based on the new system of multi-channel and highly specific analysis. A highly sensitive in situ imaging analysis method will be constructed to achieve the real-time, nondestructive, and fast multi-component simultaneous analysis, which makes up for the deficiencies as the research object for cell populations and single emission spectroscopy.

单细胞分析作为细胞微环境研究的重要途径，能够反应群体细胞因随机生化反应引起的细胞个体差异性，获得反映细胞生理状态和过程更准确和全面的信息。全无机钙钛矿纳米晶体因其具有优异的光学性能而成为诸多领域的研究焦点。本项目从制备超亲水钙钛矿纳米晶体材料出发，聚焦多波段钙钛矿纳米晶体合成方法、表面功能化修饰和细胞成像等基础问题，探索类核壳结构亲水性多波段钙钛矿量子点在单细胞体内多元成像分析新方法。通过调控核心元素种类、反应条件以及（带电）亲疏水聚合物类型，研究水相中多波段钙钛矿量子点荧光探针的光学性能、亲疏水能力及生物相容性等，结合生物成像技术，实现单细胞体内多元原位成像分析，研发多通道和高特异性的分析检测新体系，构建高灵敏的原位成像分析方法，实现实时、无损、快速的多组分同时分析，弥补以细胞群体作为研究对象和单发射光谱学的不足。

生物相容性好的探针材料是构建细胞微环境检测的重要前提。本项目基于简单的合成技术从制备水溶性钙钛矿纳米探针、碳基荧光纳米探针以及新型的电化学功能纳米复合材料出发，成功的构建了一系列新颖的荧光、电化学、光电化学传感平台并将其用于细胞中分析物的检测，实现了被测物质的高灵敏、高选择性检测；并运用两亲性中长链有机配体研究探索了钙钛矿水相稳定性与外在保护配体分子结构之间的关系，调控了钙钛矿纳米晶体在稀释条件下维持水相稳定的条件，拓展了钙钛矿纳米材料作为荧光探针在分析领域的应用。另外，我们还基于上述合成的纳米材料性能将其用于传感、催化及环境污染物降解等研究领域，在此基础上均取得了良好的研究成果。