一个新的控制水稻花药发育基因的功能和表达分析

Anther is the most important organ in the rice male reproductive and development process. Tapetum plays an important role in the process of rice anther development. It supports pollen wall formation and the subsequent pollen development, while in the late stage it is crucial for the development of fertile pollen that the Tapetum degenerates, what is considered to be triggered by programmed cell death (PCD). However, the molecular basis regulating tapetum PCD remains poorly understood. In this study, based on the research of the rice male sterile mutant which named tapetum degeneration retardation (tdr), a downstream gene OsADF regulated by TDR have been isolated by molecular biology technology. To better understand the role of OsADF during pollen development, OsADF gene will be isolated from rice and its expression patterns and functions will be analyzed. This research will clarified the gene regulation network of tapetum development and anther development at the molecular level, and will provide more theoretical support for the molecular mechanism of plant male sterility and signaling pathways.Also it is expected to create new male sterility materials for hybrid rice breeding.

水稻雄性生殖发育过程中最重要的器官是花药。绒毡层细胞发育对花药的发育起着重要的作用。在花粉发育后期，绒毡层细胞经历一个细胞程序性死亡的过程完成降解，为花粉外壁的形成以及花粉的成熟提供必须的营养物质。但绒毡层细胞在整个小孢子发育过程中如何逐渐消亡以及如何参与到小孢子发育的分子机制并不清楚。有研究表明水稻TDR基因在水稻花药绒毡层降解和发育调控过程中发挥重要的作用，我们运用ChIP-sequencing技术初步寻找到一个受TDR基因调控的下游基因OsADF，本研究将利用遗传学方法验证OsADF和TDR的调控关系，并对OsADF基因进行功能分析和表达模式分析，在分子水平上阐明绒毡层发育与花药发育的基因调控网络，为植物雄性不育的分子机理和信号通路提供更多的理论支持，也有望创制新型雄性不育材料供育种应用。

水稻雄性生殖发育过程中最重要的器官是花药,绒毡层细胞发育对花药的发育起着重要的作用。水稻TDR基因在水稻花药绒毡层降解和发育调控过程中发挥重要的作用，我们利用染色体免疫沉淀技术验证得到一个受TDR直接调控的下游基因ADF。生物信息分析发现ADF属于一个典型的水稻F-box蛋白，定位在细胞膜上；基因功能研究表明ADF基因在水稻中低水平的表达影响了绒毡层细胞的降解和小孢子的发育，从而影响了花药的育性；表达模式分析发现，ADF表达开始于小孢子时期并持续表达，空泡化时期表达最高；同时通过原位杂交发现，ADF特异表达于绒毡层和小孢子上。本项目在分子水平上为阐明绒毡层发育与花药发育的基因调控网络奠定了基础。