miR-132/MeCP2在孕晚期睡眠剥夺致子代鼠行为和生物节律紊乱中的作用及其机制研究

Sleep is a basic necessity for maintaining racial continuation and individual survival. However, two thirds of women in pregnancy encounter one or more sleep disorders such as shorten sleep time, lower quality and awakening more. Especially in late pregnancy, the incidence of sleep disorders rises greatly. Maternal sleep deprivation has a serious impact to the behavior, learning and memory of the offspring. But so far it is still a lack of system research about the effect and its mechanism of sleep deprivation during late pregnancy on offspring's psyche, behavior and the biological rhythm. In our preliminary study, we found that with sleep deprivation during late pregnancy the offspring showed hyperactivity, decreased excrement, and weight increase, sleep derive decrease, accompanied by the increasing expression of miR-132 and the decreasing expression of MeCP2 in the suprachiasmatic nucleus. Therefore, in this study we will apply gene transfection, RNA interference and single cell patch clamp technology to investigate the influence of sleep deprivation during late pregnancy on offspring mice's behavior and biological rhythm, and explore the functions of miR-132 and MeCP2 in it. This study will identify the functions of miR-132 and MeCP2 in biological rhythms and behavioral alterations of the offspring mice with maternal sleep deprivation during late pregnancy and its regulatory mechanism, and provide important information relevant to the influence of sleep disorders of pregnant women on the fetus, so as to protect the psychological health of pregnant women and newborns.

睡眠是生命活动所必须的过程，对种族延续和个体生存具有重要意义。然而，三分之二的女性在妊娠期会出现睡眠时间缩短、质量下降、觉醒次数增多等睡眠问题，特别在孕晚期睡眠障碍的发生率大为提高，对子代的行为及学习记忆均产生严重影响。但迄今为止尚缺乏孕晚期睡眠剥夺对子代的精神、行为以及生物节律的影响及其机制的系统研究。我们在预实验中发现睡眠剥夺孕鼠的子代出现活动过多、粪便数量减少、体重增加；睡眠驱动力降低，睡眠自稳态系统改变；伴有SCN中miR-132表达上调及MeCP2表达下调。本研究拟应用基因转染、RNAi、脑片膜片钳等技术研究孕晚期睡眠剥夺对子代鼠行为及生物节律的影响，探讨miR-132及MeCP2在其中发挥的作用。本研究将明确miR-132和MeCP2在孕晚期睡眠剥夺的子代小鼠的生物节律及行为改变中的作用及其调控机制，为孕妇睡眠障碍对胎儿以后的影响提供理论依据和实验基础，保护孕妇和新生儿的健康。

睡眠是生命活动所必须的过程，对种族延续和个体生存具有重要意义。然而，三分之二的女性在妊娠期会出现睡眠时间缩短、质量下降、觉醒次数增多等睡眠问题，特别在孕晚期睡眠障碍的发生率大为提高，对子代的行为及学习记忆均产生严重影响。但迄今为止尚缺乏孕晚期睡眠剥夺对子代的精神、行为以及生物节律的影响及其机制的系统研究。本项目旨在从母体睡眠剥夺后子代SCN脑区神经元发育、电生理特性和分子表达的角度，探讨孕晚期睡眠剥夺对子代小鼠的生物节律及行为改变及其调控机制，并分析孕期睡眠障碍对子代情绪行为和生物节律的关系。首先构建了孕晚期睡眠剥夺模型，进行了子代鼠行为学、脑电及睡眠节律检测，结合前期实验研究，应用脑片膜片钳、基因转染、RNAi等技术研究孕晚期睡眠剥夺对子代鼠行为及生物节律的影响，探讨miR-132及MeCP2在其中发挥的作用。本研究为探究孕期睡眠障碍对子代的影响，并为保护孕妇和新生儿的健康提供理论依据和实验基础。