TSC2/mTOR信号通路在羔羊骨骼肌细胞生长中的调节机理

TSC2/mTOR signaling pathway plays an important role in regulating cell growth by integrating nutrients and growth factors signals, exploring the roles of this signaling pathway in regulating the skeletal muscle growth of goat kid, is of a vital significance on the improvement of reduced body weight in weaning kid. Using suckling kids of Xinong Saanen dairy goats as the experimental animals, which will be fed hay at two different ages, respectively, growth performance will measured, and the real-time qPCR will be performed to determine the gene expression of TSC2/mTOR signaling pathway in skeletal muscle. Based on the expression characteristics of genes involved in TSC2/mTOR signaling pathway, the key genes which could regulate goat kid growth will be suggested. Using the established skeletal muscle cell lines, which will be treated by specific inhibitor of mTOR following TSC2 overexpression and RNA interference approaches to confirm the roles of TSC2 in regulating genes expression of mTOR signaling pathway, the mechanism of the skeletal muscle growth mediated by the genes involved in TSC2/mTOR signaling pathway will be explorded preliminarily. The outcomes will provide experimental and theoretical basis for the research of the growth regulation in dairy goat kid, and have practical significance on optimization and application of daiy goat kid supplementary feeding scheme.

TSC2/mTOR信号通路通过整合营养与生长因子信号在调节细胞生长中具有重要作用，研究该信号通路在羔羊骨骼肌生长中的调节作用对缓解断奶羔羊体重减轻具有重要意义。本项目以西农萨能羊羔羊为研究对象，在个体水平上通过对羔羊分别实施两种干草补饲方案，测定生长性能，同时采用实时荧光定量PCR技术测定羔羊骨骼肌中TSC2/mTOR信号通路基因表达量并分析不同干草补饲方案对TSC2/mTOR信号通路基因的表达特征，获得影响羔羊生长的关键基因。在细胞水平上对建立的羔羊骨骼肌细胞系首先采用TSC2过表达或RNA干扰技术，然后用mTOR特异性抑制剂处理，验证TSC2对mTOR信号通路基因的表达调控功能，初步探明TSC2/mTOR信号通路在羔羊骨骼肌细胞生长中的调节机理，为奶山羊羔羊生长调控研究奠定实验和理论基础，对哺乳期羔羊补饲方案的优化和应用具有实践指导意义。

TSC2/mTOR信号通路通过整合营养与生长因子信号在调节骨骼肌细胞生长中具有重要作用，研究该信号通路在羔羊骨骼肌生长中的调节作用对缓解断奶羔羊体重减轻具有重要意义。本项目以西农萨能奶山羊羔羊为研究对象，在个体水平上对羔羊实施两种干草补饲方案，结果显示，早期（2周龄）补饲苜蓿干草能够缓解断奶对羔羊饲料干物质采食量和平均日增重的影响，能够促进瘤胃乳头发育；获得并分析了骨骼肌组织中TSC2/mTOR信号通路基因的表达特征。利用建立的羔羊原代骨骼肌细胞系，借助过表达或RNA干扰技术，通过mTOR特异性激动剂和抑制剂处理，从细胞水平上明确了TSC2基因在骨骼肌细胞增殖与分化中的重要功能，阐释了TSC2/mTOR信号通路在骨骼肌细胞生长中的调节机理。.项目实施期间发表中文核心期刊论文2篇，SCI收录论文1篇。协助培养在读硕士生1名，培养在读硕士生1名。