囊泡运输参与盐腺泌盐过程的机理研究

Limonium bicolor Kuntze (Plumbaginaceae) is a typical exo-recretohalophyte (a halophyte that can excrete the salts that were accumulated in the leaves to the outside) that has a typical salt excretory structure in the epidermis called salt gland. Under saline conditions, L. bicolor can maintain intracellular ionic homeostasis and osmotic stability by directly secreting excess salts accumulated in the leaves onto the leaf surface. There is evidence that vesicle transporting may be involved in the secreting process of salt glands, but the mechanism is unclear. In this study, vesicle transporting invovled in secreting salt of salt glands was studied by cell biology, expression analysis of differential genes and mutant screening in L. bicolor, in order to clarify the secreting mechanism of salt glands.

二色补血草 [Limonium bicolor (Bunge) O. Kuntze]是白花丹科的泌盐盐生植物，其抗盐机制是通过多细胞盐腺(salt glands)将体内过多的盐分泌到体外，从而维持体内的离子平衡和渗透压稳定。有证据显示囊泡运输可能参与了盐腺泌盐过程，但其机理尚不清楚。本研究拟以二色补血草为材料，通过细胞生物学、差异基因表达分析以及突变体筛选等方法研究囊泡运输在盐腺泌盐过程中的作用，为阐明泌盐植物的盐腺泌盐机制打下基础。

二色补血草 [Limonium bicolor (Bunge) O. Kuntze]是白花丹科的泌盐盐生植物，其抗盐机制是通过多细胞盐腺(salt glands)将体内过多的盐分泌到体外，从而维持体内的离子平衡和渗透压稳定。有证据显示囊泡运输可能参与了盐腺泌盐过程，本研究通过囊泡化抑制剂BFA可抑制二色补血草盐腺泌盐的结果，推测囊泡运输参与二色补血草盐腺的泌盐过程；从泌盐盐生植物二色补血草中克隆了3个与囊泡运输和泌盐相关的基因，并对其进行了生物信息学分析，检测了上述候选基因在NaCl处理条件下的表达情况；构建了这3个基因的各种表达载体（过表达载体、Crispr载体)，转化野生型拟南芥和二色补血草。获得了转基因二色补血草的T1代植株，现在正在筛选纯合株系，以验证候选基因在二色补血草盐腺泌盐中的作用。获得了转基因拟南芥纯合株系和突变体纯合植株，生理结果初步表明候选基因可能与转基因拟南芥的耐盐性相关。