联萘-锌配合物选择性识别氨基酸的荧光传感器合成与应用

With the developing recognition and detection technology of fluorescence, the research about amino acids which are the basic unit of protein becomes a hot topic. However, by researching of fluorescent probe about identification of amino acids, there are some problems such as low sensitivity、 interfering with similar structures of detecting、 low enantioselectivity of chiral amino acids and so on. So, we intend to adopt binaphthyl-zinc complexes as acceptors to construct the sensor and exploit their potential in sensing amino acids. When we introduce imine groups into Binaphthyl system, On the one hand, that will be easy complex with metal ions; on the other hand, that will increase conjugated system of binaphthyl molecular, and will make a big red shift of fluorescence emission spectra. By the principle of some part of fluorescence peak be enhanced and some part be quenched to design ratio fluorescent sensor. The results of this project will further expand an application of binaphthyl-metal complexes; provide a valuable theoretical basis for design high enantioselective and sensitive fluorescence sensor to detect amino acids.

随着荧光识别及检测技术的不断发展，近年来对生物功能大分子蛋白质的基本组成单位-氨基酸的识别研究逐渐成为热点。然而在基于氨基酸分子识别的荧光探针研究中，普遍存在灵敏度低、结构相似化合物之间相互干扰、对同一组氨基酸的手性对映选择性相对较低等问题。本项目拟采用手性联萘基团结合锌离子配合物为受体，研究其在选择性识别氨基酸离子方面的应用潜力。通过在联萘分子体系中引入亚胺基，一方面使其更好地与金属离子配位，另一方面增大联萘基团分子的共轭体系，从而使荧光基团的发射光谱有较大红移。当荧光指示剂被特定氨基酸取代后部分荧光增强，另一部分荧光淬灭，依据此原理可构建比率型荧光探针。本项目研究成果将进一步拓展联萘金属配合物的应用领域, 对于设计其他新型的检测氨基酸对映选择性传感器及开发高灵敏度的对映选择性检测技术提供有价值的理论基础。

本项目以荧光识别染料为检测母体，通过引入具有功能性手性识别基团使荧光识别检测技术在生命物质-氨基酸的识别上有很好的应用。 特别是联萘酚轴手性荧光探针母体为研究对象，通过引入不同的官能团如苯并噻唑等，使新设计出的荧光探针对氨基酸中的精氨酸有很好的识别效果。同时，本项目还进行了针对苯并噻唑为功能团设计了一系列其他类型的荧光探针，同样获得了满意的荧光检测识别的效果。通过一系列的实验数据分析验证了荧光识别及检测技术可以作为一个新的热点在生命大分子中对手性物质及一般金属物质进行高选择性高灵敏度的识别应用。通过本项目的支持已有若干个成果被SCI高影响力杂志报道。