黄河流域甘肃段土壤微生物群落结构对重金属污染的适应机理研究

Gansu reach of the Yellow River, located in the upstream of the Yellow River, which is mainly water source conservation areas. But now sediments and water contamination rises year by year because of population density, industrialization and urbanization. Heavy-metal as one of major contamination has toxic effects on both humans and environmental organisms. Reduce metal contamination has caused widespread concern and becomes an essential step of environmental remediation of Gansu reach. In this study, both functional gene array (GeoChip 4.0) and 454 pyrosequencing of 16S rRNA genes will used to investigate microbial communities structural and functional shifts, analyze the ecosystem function network and describe survival strategies in heavy metal polluted habitats from Yellow River sediments. Further study to build metal bioremediation Gene resource pool, screen microbial that is able to remedy metal contamination from soil sediment and elucidate the molecular mechanism. All the results would provide useful information and powerful approach for metal bioremediation processes and ecological environment construction of western regions.

黄河流域甘肃段位于黄河上游，是黄河重要的水源涵养补给区。随着流域人口密度增加，工业化和城镇化的推进，土壤和水体的污染逐年上升。作为主要污染物之一的重金属会对人畜健康造成不可逆损害，安全有效的修复重金属污染已成为亟待解决的科学问题。本项目拟采用454测序和GeoChip技术，研究黄河流域上游甘肃段不同程度重金属污染的土壤中的微生物群落多样性及其在重金属胁迫下的群落结构和功能类群特征的演化趋势，描述微生物群落的生态功能网络，揭示重金属胁迫下微生物的响应机制，阐明微生物抗重金属污染的机理，为应用微生物修复黄河流域重金属污染提供理论支持。挖掘一批具有潜在应用价值的重金属污染治理功能菌株并和建立功能基因资源库，为西部地区的生态环境建设提供科学依据。

黄河流域甘肃段位于黄河上游，是黄河重要的水源涵养补给区。随着流域人口密度增加，工业化和城镇化的推进，土壤和水体的污染逐年上升。作为主要污染物之一的重金属会对人畜健康造成不可逆损害，安全有效的修复重金属污染已成为亟待解决的科学问题。本项目采用宏基因组测序技术，研究黄河流域上游甘肃段不同程度重金属污染的土壤中的微生物群落多样性及功能类群特征，描述微生物群落的生态功能网络，揭示重金属胁迫下微生物的响应机制，阐明微生物抗重金属污染的机理，为应用微生物修复黄河流域重金属污染提供理论支持。挖掘一批具有潜在应用价值的重金属污染治理功能菌株和功能基因，为西部地区的生态环境建设提供科学依据。