基于微腔谐振的毛细管内壁捕获抗体固载状态表征方法研究

The characterization method of the immobilized state of the immune antibody is very important for the selection of trimmings, the recycling of enzyme immobilized and the efficiency of sample analysis, which is an important basis for modification and preparation of capillary enzymatic microreactors..This project intends to use resonant coupling in fiber taper-capillary microcavity as the core technology. Fiber taper microstructure and capillary microchannel are employed to establish the microenvironment of capture antibody of alpha fetal protein, at the same time to characterize the immobilization state of antibodies on the inner surface of capillary. The characteristic of WGM spectral signal excited by fiber taper-capillary coupling structure are studied and analyzed. The functional relationship between the immobilization surface variation-spectra parametric model and the characteristic parameters of antibody microfluidic immobilization conditions will be established, hoping to provide a new idea for the optimization of antibody immobilization conditions for capillary microreactors. .The research of this project can solve the key scientific problems of characterization of antibody immobilization state in capillary surface. The resonance coupling technique based on fiber cone-capillary microcavity is used to systematically study the immobilization process of antibody on the capillary inner surface, which has strong theoretical research value and broad application prospects. The implementation of this project can provide reference for the detection of capillary immunological functional modification, which promote development of optical fiber sensing technology on bio-detection field.

免疫分析抗体的固载状态表征作为毛细管酶促微反应器修饰制备的重要依据，对修饰物选择、酶固定循环利用、样本分析效率至关重要。.本项目以光纤锥-毛细管微腔谐振耦合为核心技术，利用光纤锥微纳结构与毛细管微流道建立肿瘤标志物甲胎蛋白捕获抗体的固载微环境，研究光纤锥-毛细管耦合结构激发的回音壁模式光谱信号特征，表征毛细管内表面抗体固载状态，建立固载表面变化光谱参数模型与抗体微液流固载条件特征参数之间的函数关系，以期为毛细管微反应器抗体固载条件优化研究提供新思路。.本项目旨在解决毛细管内表面免疫分析抗体固载状态表征的关键科学问题，基于光纤锥-毛细管微腔谐振耦合技术系统研究抗体微管内表面固载过程，具有很强的理论研究价值及广泛的应用前景。本项目的实施可为毛细管免疫功能化修饰检测领域提供借鉴，促进光纤传感技术在生物检测领域的发展。

本项目从毛细管内壁抗体固载状态特性出发，在毛细管微反应器应用背景下的微管回音壁耦合谐振激发原理基础上，发展以光纤微结构-毛细管微腔谐振耦合结构为核心的检测理论和方法。本项目创新性地提出光纤楔形端面和光纤锥尖探针微结构的新型谐振耦合模型，结合光束传播法和时域有限差分法对模型进行了仿真计算和结构优化，建立了一整套基于研磨和CO2激光熔融拉锥工艺的毛细管微反应器和光纤微结构耦合器的制备方法，搭建了基于光纤微结构-毛细管微腔谐振模式耦合光谱采集系统、显微成像系统、传感检测装置和微液流反应器，通过对谐振光谱信息的提取实现了毛细管微反应器谐振耦合检测。本项目所提出的一系列耦合结构和谐振激发方法对毛细管微反应器光学传感结构的设计和性能提升具有普遍意义和推广价值，是对微光学谐振腔研究的重要拓展，在毛细管免疫功能化修饰检测领域具有重要应用前景。相关成果发表8篇学术论文，其中包括SCI检索论文3篇，EI论文2篇，中文核心论文3篇；申请中国发明专利4项。