基于静电纺丝的明胶-玉米醇溶蛋白复合纳米纤维载运疏水性功能分子的控释机理

The electrospun hydrophilic gelatin nanofiber delivery systems have very promising applications in food industry, but the hydrophobic bioactives encapsulated within gelatin nanofibers can not be effectively released in the gastrointestinal environment due to the burst release effect. In this research project, we will construct gelatin-zein hybrid nanofibers based on different ordered structures via molecular self-assembly to deliver and release typical hydrophobic bioactives. We will study the oscillating stability of Taylor Cone to analyze the regulating patterns of protein self-assembly driving force, in order to understand the formation mechanism; we will study the delivery process of hydrophobic bioactives to analyze the mechanical performance, wetting properties, thermal behaviors and molecular interactions of nanofibers, and locate the delivery zones, in order to illustrate the delivery mechanism; we will study the release behaviors under simulated gastrointestinal conditions to establish the release kinetics models, and analyze the influence of the release behaviors of different ordered structures on the antimicrobial and antioxidant activities, in order to reveal the release mechanism; we will combine the studies of the formation, delivery and release mechanisms to fully explain the controlled release mechanism of hydrophobic bioactives from electrospun gelatin-zein hybrid nanofiber delivery systems. The results of this research project will not only lay a theoretical foundation for the controlled release from food-grade electrospun nanofiber delivery systems, but also provide a practical guideline for solving the effective release problems of hydrophilic nanofibers in the gastrointestinal environment.

基于静电纺丝的亲水性明胶纳米纤维载运体系应用前景良好，但由于突释效应明显，难以在胃肠消化环境中有效缓释。本项目拟基于不同有序结构的分子自组装，构建各种明胶-玉米醇溶蛋白复合纳米纤维，载运控释疏水性功能分子；通过泰勒锥振荡规律的稳定性研究，分析蛋白自组装驱动力的分子调控规律，解析形成机制；通过疏水性功能分子的载运过程研究，分析宏观性能变化、润湿性质转变规律、热力学行为变化及分子相互作用，定位载运区域，阐明载运机制；通过胃肠消化模型的释放行为研究，建立释放动力学模型，分析不同纤维有序结构的释放行为对抑菌、抗氧化等活性变化规律的影响，明确释放机制；综合分析，全面揭示明胶-玉米醇溶蛋白复合纳米纤维载运疏水性功能分子的控释机理。项目研究结果不但为基于静电纺丝的食品级纳米纤维载运体系控释疏水性功能分子研究奠定理论基础，而且对于解决亲水性纳米纤维在胃肠消化环境中的有效释放问题也具有重要的实践指导意义。

基于静电纺丝的亲水性明胶纳米纤维载运体系应用前景良好，但由于突释效应明显，难以在胃肠消化环境中有效缓释。本项目构建了各种明胶-玉米醇溶蛋白复合纳米纤维，载运控释疏水性功能分子；分析了蛋白自组装驱动力的分子调控规律，解析明胶-玉米醇溶蛋白复合纳米纤维的形成机制；通过疏水性功能分子的载运过程研究，分析了宏观性能变化、润湿性质转变规律、热力学行为变化及分子相互作用，阐明明胶-玉米醇溶蛋白复合纳米纤维的载运机制；通过胃肠消化模型的释放行为研究，分析了纤维有序结构的释放行为对生物活性变化规律的影响，明确明胶-玉米醇溶蛋白复合纳米纤维的释放机制；最后综合分析，全面揭示明胶-玉米醇溶蛋白复合纳米纤维载运疏水性功能分子的控释机理。项目研究结果为基于静电纺丝的食品级纳米纤维载运体系控释疏水性功能分子研究奠定理论基础，对于解决亲水性纳米纤维的有效释放问题也具有重要指导意义。