基于DNA条形码探讨长江口及其邻近水域鱼类浮游生物的分类与群落结构变化

The Yangtze estuary and adjacent waters are of high ecological value. The classification of ichthyoplankton and variation of the ichthyoplankton community in this water are also very important to the fisheries. In the present study, both the morphological characters and the DNA barcoding are used in order to accurately identify the ichthyoplankton. The aims of the present study are: founding the DNA barcoding database of the ichthyoplankton in the Yangtze estuary and adjacent waters and extending the DNA barcoding information of marine fish in China; finding the fast and effective morphological classification characters and developing the multi-identification methods of ichthyoplankton; indicating the composition and diversity of ichthyoplankton species in the Yangtze estuary and adjacent waters, as well as the relationship between the community and the environmental factors. The results of the present study would be useful for the identification of the ichthyoplankton, the fisheries resources management and protection.

长江口及其邻近水域具有重要的生态地位，该水域的鱼类浮游生物分类及群落结构变化研究具有重要意义。本项目将利用传统形态学和DNA条形码相结合的方法，对长江口及其邻近水域这一典型区域的鱼类浮游生物进行准确鉴定，从而：建立长江口及其邻近水域鱼类浮游生物的DNA条形码数据库，丰富我国海洋鱼类DNA条形码信息；发掘快速、稳定、有效的鱼类浮游生物的形态学分类特征，为探索分子与形态相结合的综合分类方法、补充发展鱼类浮游生物的分类体系奠定基础；揭示鱼类浮游生物在长江口及其邻近水域的物种组成、丰度变化及不同季节、不同年度的群落结构变化，及它们与环境因子的响应机制，阐明长江口及其邻近水域对于鱼类产卵和育幼的意义，为长江口河口生态系统及相关保护区的鱼类资源保育及海洋生态保护提供参考。

长江口及其邻近水域具有重要的生态地位，该水域的鱼类浮游生物分类及群落结构变化研究具有重要意义。本项目利用传统形态学和DNA条形码相结合的方法，实现了对长江口及其邻近水域这一典型区域的鱼类浮游生物的准确鉴定；建立了长江口及其邻近水域鱼类浮游生物的DNA条形码数据库，丰富了我国海洋鱼类条形码信息；从线粒体基因组、转录组等基因组层面研究了长江口及其邻近水域鱼类浮游生物的遗传多样性；从长时间尺度揭示了春季、秋季鱼类浮游生物在长江口及其邻近水域的物种组成、丰度变化、群落结构变化，及其与环境因子的响应机制。本项目成果对于阐明长江口及其邻近水域的鱼类产卵和育幼功能具有重要意义，为长江口河口生态系统及相关保护区的鱼类资源保育及海洋生态保护提供了依据。