一个功能未知的lncRNA SPAG5-AS1促进宫颈癌增殖的作用机制研究

SPAG5 - AS1 is our previous application lncRNA chip screening and remarkably high expression in cervical cancer, the function of the unknown a long chain of non-coding RNA. Early discovery, silence SPAG5 - AS1 can significantly inhibit cervical cancer cell proliferation, promote apoptosis of bioinformatics analysis and experimental results are prompt SPAG5 - AS1 can combine with hnRNP - H and trans downstream target A specific regulation. In the early stage of the existing results of this research is based on the proposed expression/silence strategy, demonstrate SPAG5 - AS1 proliferation role in cervical cancer; RIP, CHIP technology fully demonstrate SPAG5 - AS1 by recruiting hnRNP -h trans control A specific molecular mechanisms. About SPAG5 - AS1 the effect and mechanism of the tumor was not reported, so has the source of innovation, this study will help to clarify the origin and development of cervical cancer mechanism, and provide new clues for cervical cancer treatment and potential targets.

SPAG5-AS1是我们前期应用lncRNA芯片筛选获得的、显著高表达于宫颈癌中、功能未知的的一条长链非编码RNA。前期发现，沉默SPAG5-AS1可显著抑制宫颈癌细胞的增殖促进凋亡，生物信息学分析和实验结果均提示SPAG5-AS1可以与hnRNP-H结合进而反式调控下游靶标A-Raf。本研究在已有的前期结果基础上拟采用过表达/沉默策略，论证SPAG5-AS1在宫颈癌中增殖中的作用；应用RIP、CHIP等技术充分论证SPAG5-AS1通过招募hnRNP-H反式调控A-Raf的分子机制。有关SPAG5-AS1在肿瘤中的作用和机制未见报道，因此本研究具有源头创新性，将有助于阐明宫颈癌发生发展的机制，为宫颈癌治疗提供新线索和潜在靶标。

SPAG5-AS1是我们前期应用lncRNA芯片筛选获得的、显著高表达于宫颈癌中、功能未知的的一条长链非编码RNA。前期发现，沉默SPAG5-AS1可显著抑制宫颈癌细胞的增殖促进凋亡，生物信息学分析和实验结果均提示SPAG5-AS1可以与hnRNP-H结合进而反式调控下游靶标A-Raf。本研究在已有的前期结果基础上拟采用过表达/沉默策略，论证SPAG5-AS1在宫颈癌中增殖中的作用；应用RIP、CHIP等技术充分论证SPAG5-AS1通过招募hnRNP-H反式调控A-Raf的分子机制。有关SPAG5-AS1在肿瘤中的作用和机制未见报道，因此本研究具有源头创新性，将有助于阐明宫颈癌发生发展的机制，为宫颈癌治疗提供新线索和潜在靶标。