基于ACE/ACE2轴和VEGF-Dll4/Notch通路研究针刺干预脑梗死侧枝循环建立的分子机制

Cerebral infarction cerebral collateral circulation including early vasodilation and subsequent angiogenesis. On the one hand was found in brain tissue RAS system has a dual effect on cerebral blood flow regulation, RAS addition to promoting vasoconstriction of the classic ACE → AngII axis, there is also its antagonistic ACE2 → Ang 1-7 axis; on the other hand study found that VEGF and Dll4 / Notch signaling pathway in angiogenesis plays an important role in this study as the research object MCAO rats after cerebral infarction vasomotor and angiogenesis.main line, from ACE- RAS system AngⅡ / ACE2- Ang 1-7 axis and VEGF-Dll4 / Notch pathway angle, laser Doppler, gait analysis, confocal laser scanning microscopy, real-time quantitative PCR, immunohistochemistry, Western-blot ELISA, etc.advanced technology and methods, dynamic visual observation of rat blood flow, cerebral angiogenesis-related changes of neurological deficit and the passage of.key indicators and acupuncture intervention effect after cerebral infarction, cerebral infarction explore among the points after acupuncture intervention mechanism of collateral circulation.

脑梗死发生后脑侧支循环建立包括早期的血管舒张与随后的血管新生。一方面研究发现脑组织的RAS系统在脑血流的调节中具有双重效应，RAS除了促进血管收缩的经典ACE→AngII轴之外，还存在与其拮抗的ACE2→Ang 1-7轴；另一方面研究发现VEGF和Dll4/Notch信号传导途径在血管新生中扮演着重要的角色，本研究以MCAO大鼠为研究对象，以脑梗死后血管舒缩和血管新生为主线，从RAS系统的ACE- AngⅡ/ACE2- Ang 1-7轴和VEGF-Dll4/Notch通路的角度，应用激光多普勒、步态分析、激光共聚焦显微镜检测、实时荧光定量PCR、免疫组化、Western-blot酶联免疫等先进的技术和方法，动态直观观测大鼠脑梗死后血流改变、脑血管新生、神经功能缺损和上述通路关键指标的相关变化规律和针刺干预效应，探讨针刺人中穴对脑梗死后侧支循环的干预机制。

脑梗死发生后脑侧支循环建立包括早期的血管舒张与随后的血管新生。一方面研究发现脑组织的RAS系统在脑血流的调节中具有双重效应，RAS除了促进血管收缩的经典ACE→AngII轴之外，还存在与其拮抗的ACE2→Ang 1-7轴；另一方面研究发现VEGF和Dll4/Notch信号传导途径在血管新生中扮演着重要的角色，本研究以MCAO大鼠为研究对象，以脑梗死后血管舒缩和血管新生为主线，从RAS系统的ACE- AngⅡ/ACE2- Ang 1-7轴和VEGF-Dll4/Notch通路的角度，应用激光多普勒、步态分析、激光共聚焦显微镜检测、实时荧光定量PCR、免疫组化、Western-blot酶联免疫等先进的技术和方法，动态直观观测大鼠脑梗死后血流改变、脑血管新生、神经功能缺损和上述通路关键指标的相关变化规律和针刺干预效应，探讨针刺人中穴对脑梗死后侧支循环的干预机制。结果发现电针人中穴可以可缓解脑血管的痉挛状态，促进脑血管舒张，增加脑血流，降低脑梗死容积，促进血管新生。其作用机制是针刺对急性脑缺血大鼠ACE- AngII/ACE2- Ang(1-7)轴及VEGF-Dll4/Notch通路有良性调整作用。该研究为针刺治疗脑梗死的临床推广应用提供科学的实验依据，并在理论上丰富和深化脑梗死发生发展的“微血管枢纽学说”。