微囊藻毒素-LR检测用氮杂石墨烯基光电化学适配体传感器研究

It is of great importance for high performance determination of microcystins-LR in trace level, which was meaningful for environmental water pollution control, ecological safety and human health. In order to dissolve the disadvantages of the existing microcystins-LR detection method, such as low sensitivty, selectivity and high cost, in this project, starting with the synthesis of nitrogen doped graphene based heterojunction, the photoelectrochemical aptasensor for determination of microcystins-LR in real samples was fabricated by the combination of photoelectrochemical techanology to method of the aptasensor. In order to improve the sensitivity of the photoelectrochemical sensor, the photoactivity of semiconductor was sensitized by the intergrating of nitrogen doped graphene. Furthermore, by the combination of the photoelectrochemical technology with electrochemical aptasensing method, a sereis of photoelectrochemical aptasensor for sensitive, selective, and low cost determination of trace level microcystins-LR was fabricated, which can break through the bottleneck of the existing microcystins-LR detection method of high cost, poor stability and low sensitivity. Therefore, the implementation of this project has great academic theory value and application prospect.

发展高性能的微囊藻毒素-LR检测方法对控制水体富营养化污染、保障生态安全及维护人类健康具有重要意义。本项目针对现有微囊藻毒素-LR检测方法灵敏度低、选择性和稳定性差及成本高等问题，拟以制备氮杂石墨烯基异质结为出发点，利用灵敏度高、响应快速的光电化学技术，结合适配体传感方法的高特异识别性能，构建光电化学适配体传感器应用于实际样中微囊藻毒素-LR的高性能检测。项目研究通过设计制备具有良好光电化学活性的氮杂石墨烯基异质结，改善半导体材料的光电化学活性，提高光电化学传感界面的灵敏度；进一步与适配体传感方法的高特异性结合，建立光电化学适配体传感方法，提高微囊藻毒素-LR检测的灵敏度和选择性，并降低检测成本。该研究可突破现有微囊藻毒素-LR检测技术中成本高、稳定性差及灵敏度低的瓶颈，具有较高的学术理论价值和应用前景。

发展高性能的微囊藻毒素-LR检测方法对控制水体富营养化污染、保障生态安全及维护人类健康具有重要意义。针对现有微囊藻毒素-LR检测方法灵敏度低、选择性和稳定性差及成本高等问题，本课题设计和制备具有高光电化学活性的铋系半导体异质结，通过改善半导体的光电转换效率，进而提高传感界面的灵敏度；进一步耦合光电化学技术的高灵敏度与适配体传感方法的低成本与高选择性，充分利用光电化学适配体传感方法的优势，研制了可用于微囊藻毒素-LR高性能检测的光电化学适配体传感器，主要工作内容包括研制了一种基于NFGQDs/3DBiOI杂化空心微球的光电化学适配体传感器用于微囊藻毒素-LR的高灵敏度、选择性检测，构筑了基于CdTe-Bi2S3异质结的光电化学适配体传感界面，应用于微囊藻毒素-LR的高灵敏和高选择性检测，分别以TiO2和氮杂石墨烯-溴化氧铋纳米材料作为光阳极和光阴极基底材料，构建了一种双光电极光助型自供能传感平台。制备的传感器选择性、重现性和稳定性良好，且在实际样分析中表现出令人满意的效果。本项目研究对于提高微囊藻毒素-LR的检测性能及扩展光电化学适配体传感器的应用领域具有重要的研究意义。