Plectin对种植体周-结合上皮半桥粒结构的调控作用

Peri-implantitis is the most common complications of dental implants. The main reason is that the epithelium cuff sealing barrier between dental implant and junctional epithelium is damaged. Bone is resorbed and the osseointegration interface is destroyed because of bacteria invasion. Previous studies indicated that the hemidesmosome is the key structure for epithelium cuff sealing barrier.Studies in dermatology demonstrated that integrity of complexes of plectin and integrinα6β4 is the most important factor to maintain the stability of hemidesmosome. The mutation or deficiency of plectin can cause the disassembly of hemidesmosome and cause epidermolysis bullosa. Therefore, the hypotheses of our study is proposed: plectin may regulate the hemidesmosome between dental implant and junctional epithelium. The present project is designed to explore the regulation role of plectin on hemidesmosome between dental implant and junctional epithelium, and the role of RON signaling pathway in the regulation role of plectin, and the feasibility and validity of plectin in treatment of peri-implantitis to reattach the junctional epithelium. Our project may be beneficial to uncover the mechanism of epithelium cuff sealing barrier around dental implants and develop novel preventive and therapeutic strategies against peri-implantitis.

种植体周围炎是种植义齿修复后最常见的并发症，主要的原因是种植体颈部上皮袖口屏障被破坏，细菌侵袭造成骨吸收和骨结合界面的破坏。种植体周-结合上皮半桥粒是上皮袖口封闭的关键。皮肤病学等领域研究发现，plectin和整合素α6β4复合物是维持半桥粒稳定的最重要因素，plectin基因突变或缺失会导致半桥粒结构解体而引起皮肤大疱。因此，本项目提出假设，plectin很可能对种植体周-结合上皮半桥粒结构具有重要调控作用。本项目旨在探讨plectin对种植体周-结合上皮半桥粒结构的调控作用和RON信号通路对调控的影响，plectin在促进种植体周围炎结合上皮再附着中的可行性和有效性。该项目的成功探索，将有助于揭示种植体上皮封闭的机理，并为种植体周围炎的防治提供新的思路。

种植体周围炎是种植义齿修复后最常见的并发症,主要的原因是种植体颈部上皮袖口屏障被破坏,细菌侵袭造成骨吸收和骨结合界面的破坏。种植体周-结合上皮半桥粒是上皮袖口封闭的关键。皮肤病学等领域研究发现,plectin和整合素α6β4复合物是维持半桥粒稳定的最重要因素，plectin基因突变或缺失会导致半桥粒结构解体而引起皮肤大疱。因此，本项目提出假设，plectin很可能对种植体周-结合上皮半桥粒结构具有重要调控作用。本项目旨在探讨plectin对种植体周-结合上皮半桥粒结构的调控作用，plectin在促进种植体周围炎结合上皮再附着中的可行性和有效性。.主要研究内容为：（1）Plectin在健康种植体周-结合上皮和种植体周围炎中的表达研究；（2）Plectin对种植体周-结合上皮半桥粒结构的调控作用；（3） plectin对种植体周-结合上皮半桥粒结构组装的影响及其机制研究；（4）建立种植体周围炎大鼠模型，检测plectin在促进种植体周围炎结合上皮再附着的研究。. 研究结果：种植体周围炎时种植体周-结合上皮中plectin、integrin α6β4、RON的表达呈显著下降；脂多糖（LPS）对口腔上皮细胞（OEC）增殖无明显影响，可显著抑制OEC细胞的黏附和迁移，表明LPS可通过下调plectin、integrin α6β4、RON的表达，抑制OEC细胞的黏附和迁移；plectin通过RON途径参与种植体周-结合上皮半桥粒结构的组装。.该项目的进展有助于揭示种植体周结合上皮封闭的机理，为种植体周围炎的预防和治疗提供了新的思路。