基于模块化多肽制备功能金属纳米粒子用于微生物细胞的检测和杀灭

The effective detection and killing of microbes is important for the diagnosis and prevention of infectious diseases, water and food safety, as well as agricultural productions. In recent years, metal nanoparticles have been widely applied in the development of microbial detection and antimicrobial agents, due to their unique properties and broad-spectrum antimicrobial activities. However, the rational design of surface molecules for nanoparticle functionalization and the elucidation of interaction between these molecules and microbial cells on the nanoscale interfaces are still challenging issues. In this project, we will directly synthesize functionalized metal nanoparticles through the modularized peptides which not only have antimicrobial activities, but also can act as building blocks for the nanoparticle synthesis. By exploring the interaction and synergistic effect between modularized peptides and microbial cells on the nanoscale interfaces, the design of modularized peptides and nanoparticles will be further optimized. Based on these advances, the as-designed nanoparticles will be applied to develop high-selective, rapid, and effective methods for the microbial detection and killing in the practical samples. This project will provide a new way for developing the methods of nanoparticles-based microbial detection and killing, and facilitate understanding of the mechanisms governing the actions of nanoparticles to microbial cells.

微生物的有效检测和杀灭对于传染性疾病的诊断和防治、水体和食品安全、以及农业生产具有非常重要的意义。近年来，金属纳米粒子凭借其独特的物理、化学性质和广谱的抗菌作用，已在微生物检测和抗菌制剂的研发中获得了广泛的应用。然而，合理设计纳米粒子的表面功能化分子，并揭示功能化分子在纳米界面上对微生物细胞的作用方式，仍然是上述应用所面临的重要挑战。本项目拟采用具有抗微生物活性的模块化多肽直接制备功能金属纳米粒子，并研究多肽在纳米界面上对微生物细胞的识别和作用模式，及其与纳米粒子的协同效应。以此为基础，进一步优化模块化多肽和金属纳米粒子的设计，发展一种针对实际样品的、高效、高选择性的微生物快速检测和杀灭方法。本项目的研究工作将为发展基于功能纳米粒子的微生物检测和杀灭方法提供新的思路，同时也将有助于理解纳米粒子对微生物细胞的作用机制。