基于三维碳纳米基质支撑蛋白质印迹复合萃取材料研制及吸附机理研究

The project aims to develop a method for the preparation of novel protein imprinted extraction materials with high selectivity, high adsorption capacity, thickness control and rapid adsorption rate combined nanotechnology and surface imprinting method. The protein imprinted materials were prepared based on three-dimensional graphene/carbon nanotubes composite materials under mild conditions in aqueous solution, which solves the preparation problem of biological macromolecules involving protein imprinted polymer. Combining with solid-phase extraction and chromatography detection technology, protein imprinting separation and enrichment-high performance liquid chromatography (HPLC) online detection system was established using the novel protein imprinted polymer as extraction agent, which simplified the technology for separation, enrichment and determination of large molecules involving proteins. Combined with a variety of adsorption models, further study the adsorption mechanism of the protein imprinted solid phase extraction materilas based on three-dimensional graphene/carbon nanotube composite materials surface, which provides the theoretical foundation for the application of protein molecularly imprinted polymer.

该项目旨在结合纳米技术和表面印迹技术，优化合成条件，探索在水溶液中、温和条件下以功能化的石墨烯/碳纳米管3D复合材料为基质制备高选择性、高吸附容量、厚度可控和吸附速度快的蛋白质等生物大分子新型印迹复合萃取材料，解决目前蛋白质等生物大分子印迹聚合物制备难题。以3D石墨烯/碳纳米管表面蛋白质印迹复合萃取材料为萃取材料，结合印迹固相萃取技术和色谱检测技术，建立蛋白质在线印迹分离富集-高效液相色谱检测系统，简化蛋白质等大分子的分离、富集和分析检测工艺，实现蛋白质等大分子印迹固相萃取分离-在线快速分离检测。结合多种吸附模型，深入研究3D石墨烯/碳纳米管表面蛋白质印迹固相萃取复合材料对目标蛋白质等生物大分子的吸附行为和吸附机理，为蛋白质印迹聚合物的应用提供理论基础。

本项目以碳纳米管、氧化石墨烯和四氧化三铁纳米颗粒等为基质，结合纳米技术和表面印迹方法，优化合成条件，制备出一系列选择性高、吸附容量高和吸附速度快的蛋白质等生物大分子新型印迹复合材料，解决蛋白质等生物大分子印迹聚合物制备的难题。以蛋白质印迹材料为分离萃取剂，结合印迹固相萃取技术和现代检测技术，建立蛋白质在线印迹分离富集-快速检测系统，简化蛋白质等大分子的分离、富集和检测工艺，实现蛋白质等大分子快速分离检测。结合多种吸附模型，深入研究新型蛋白质印迹复合材料对目标生物大分子的吸附行为和吸附机理，为新型蛋白质印迹聚合材料的应用提供理论基础。该项目研究成果在国内外发表相关研究论文13篇，其中SCI收录10篇。该项目培养研究生共11名，毕业6人，在读5人；获湖南省优秀硕士毕业论文1篇，圆满完成项目的预期目标。