LncRNA uc.308影响卵母细胞成熟的机制研究

uc.308 is a highly expressed lncRNA in mouse GV oocyte, identified by LncRNA microarray in our previous study. And it is evolutionarily conserved from plants to mammals with 100% homologous to human. However, the physiological function of uc.308 in oocyte maturation remains unknown. Here, we showed that downregulation of uc.308 led delayed GVBD and decreased first polar body extrusion. With bioinformatic analysis data, we found that downregulation of uc.308 caused increased expression of Btrc, which was important for oocyte maturation. Based on these information, we design to investigate the expression patterns of uc.308 in different stages of mouse immature oocytes. And use overexpression and knockdown technology to analyse its physiological function in the process of oocyte maturation. In addition, we will explore the potential mechanism of uc.308 interaction with Btrc in the process of oocyte maturation. So far there is no study about the function of lncRNA uc.308 in oocyte maturation, this study is innovative and will provide new target for the promotion of clinical IVM technology.

uc.308是我们应用芯片技术筛选到的、差异高表达于小鼠未成熟卵母细胞中、功能未知的一条LncRNA。前期发现：①小鼠卵母细胞中uc.308表达沉默可致卵母细胞成熟异常；②生物信息学提示Btrc基因可能为uc.308分子靶标，人与小鼠uc.308序列及在Btrc基因结合序列的保守性100%；③uc.308表达沉默的卵母细胞中Btrc显著上调，而文献报道Btrc编码蛋白是影响卵母细胞成熟的重要分子，提示uc.308可能通过调节Btrc的机制而影响卵母细胞成熟。研究拟：揭示uc.308在卵母细胞中的表达规律；以表达沉默和过表达双向策略，评价uc.308在卵母细胞成熟过程中的作用；以Btrc为切入点，系统论证uc.308致卵母细胞成熟异常的分子机制。LncRNA uc.308在卵母细胞中的功能与机制目前未见报道，本研究将为阐明卵母细胞的成熟机制提供新线索，可为临床IVM技术提供潜在新干预靶标。