灯盏花自交不亲和信号转导因子ARC1与SRK的互作研究

Erigeron breviscapus, one of the most important medicinal plants in Yunnan province, has notable curative effectiveness for cardio-cerebrovascular diseases. So far, researches on the mechanism of E.breviscapus self-incompatibility are very limited. Our previous research showed that Erigeron breviscapus has typical self-incompatibility characteristics. SRK, the female determinant factor of self-incompatibility in Erigeron breviscapus, was cloned. Analysis of the temporal and spatial expression patterns of the SRK gene has been carried out and the transformation system of Erigeron breviscapus has been optimized. As a important downstream signaling of SRK, ARC1 is the key point of the research. In this study, temporal and spatial expression patterns of ARC1 and its interaction with SRK will be study. We would elucidate the following scientific issues: (1) what is the temporal and spatial expression pattern of ARC1 gene ? What is the relationship between it and self-incompatibilty? (2) what are the functions of multiple domains in ARC1? which amino acid residues specifically determine the ARC1-SRK interaction? what does the interaction patterns between ARC1 and SRK mean in the self-incompatibility of E. breviscapus. This study will have the following scientific significance: (1) understanding the function of ARC1 in the signal transduction pathway of E.breviscapus self-incompatibility. (2) providing a scientific support to molecular breeding of E.breviscapus.

灯盏花是云南最具地方特色的药用植物之一，具有良好的抗心脑血管疾病的作用。我们的前期研究表明灯盏花具有典型的自交不亲和的特性，克隆了灯盏花的自交不亲和的雌性决定因子—SRK，分析了SRK基因的时空表达模式，优化了灯盏花的转基因体系。到目前为止，关于灯盏花自交不亲和的机理研究得较少。ARC1作为SRK下游重要的信号传导分子，一直是研究的重点。本项目主要研究灯盏花自交不亲和转导因子ARC1的时空表达模式及其与SRK的作用模式特征。探讨的主要科学问题是：1）ARC1基因在灯盏花中的表达有什么特征？与自交不亲和反应用什么相关性？2）ARC1的多个结构域的特征是什么？哪些氨基酸残基决定了ARC1-SRK互作的特异性？ ARC1和SRK的互作模式的特征对自交不亲和有什么意义？本项目的科学意义：1）了解灯盏花信号传导因子ARC1在自交不亲和信号传导途径中的作用和地位；2）为药用植物灯盏花分子育种奠定基础。

灯盏花是云南最具地方特色的药用植物之一，具有良好的抗心脑血管疾病的作用。到目前为止，关于灯盏花自交不亲和的机理研究得较少。我们的研究表明灯盏花具有典型的自交不亲和的特性， ARC1作为SRK下游重要的信号传导分子，一直是研究的重点。本项目主要克隆了灯盏花的自交不亲和的信号转导因子ARC1，分析了ARC1基因的功能，它能导致拟南芥植株变矮，分蘖力弱，穗子短小，结实率低和种子瘦小。本项目进一步分析了ARC1与Exo70A1的相互作用特征。本项目的科学意义：1）了解灯盏花信号传导因子ARC1的功能、验证了ARC1与Exo70A1的相互作用，为菊科植物自交不亲和信号传导途径的解析奠定了基础；2）本文结果为研究分子育种和研究基因功能分析方法提供了重要依据。