核酶微阵列检测技术的研究

ABSTRACT: The characteristics of the ribozymes designed in the study are (1).stem III is to bind MS2 coat protein; (2) stem II structure formed by itself isincoorect, but the correct structure can be formed by the regulation region pairedwith the target sequence to generate an active hammerhead ribozyme; and (3) 3’-end is for fixing the molecule on the surface of solid phase. The ribozyme is prepared hrough molecular biological methods and fixed on the solid chemically.If an RNA or DNA that can be complementary to the regulation sequence exists inthe solution, the ribozyme will be auto-cleaved to release the stem III which isbound to the protein crosslinked to peroxidase, so the spot color decrease on the solid surface reflecting the amount of specific nucleic acid in the solution..KEY WORDS: ribozyme; allostery; nucleic acid detection

T7-RNA聚合酶体外转录制备核酶，并进一步制造成核酶微阵列。这种核酶具有可与蛋白结合的RNA，并且需要特定的核酸序列与之配对后才能自裂解。自裂解的结果使微阵列的RNA失去可与蛋白质结合的结构。用既能与RNA结合又有过氧化物歧化酶活性的融合蛋白与杂交后的⒄罅薪岷喜⒔邢陨从ΑＵ庵旨觳饧际醪灰蟊昙谴庋罚僮骷虻ィ杀镜汀