双靶向肽对力负载下成骨细胞基因差异表达影响的研究

It is a considerable mode of modifying titanium implant surface with peptides or proteins deriving from "cement line" to accelerate osseointegration, in which the effective fixation of peptides/proteins results in success. Meanwhile, the implant stability under occlusive loading is a challenge which links closely with its clinical appliance. In this project, RGD peptide will be fixed on Ti by linking with Ti-binding peptide（minTBP-1）.Osteoblasts on Ti surface modified this chimeric peptide will be exposed to mechanical loading and their biologic activities will be studied. SAGE（Serial Analysis of Gene Expression）, a high throughput method, will be applied to globally investigate the gene profiling of osteoblasts after exposure to mechanical loading. And a systemic understanding of mechanotransduction in osteoblasts under dual effects of chimeric peptide and mechanical loading will be realized with the aid of bioinformatics processes. All our work will be helpful to understanding the interaction between Ti implants and bone tissue and will provide the foundation for theory and application.

采用"粘合线"来源的多肽或蛋白修饰钛表面是提高钛种植体骨整合速率与质量的重要方式，其中多肽/蛋白固定于钛表面的方式是有效涂层的关键；同时，植入颌骨的种植体受到的力学负载挑战着其植入的稳定性。种植体涂层能否在力学加载情况下发挥作用，是其应用于临床的重要前提。本项目采用近年来兴起的利用钛表面结合肽功能序列（Ti-binding peptide, minTBP-1）固定RGD序列的连接方式，在钛表面建立"双靶向"融合肽涂层，并对其上成骨细胞加以压应力负载，探讨成骨细胞生物学活性变化；同时利用高通量基因组水平研究手段SAGE（Serial Analysis of Gene Expression）技术，从全局揭示融合肽-应力双重作用下细胞的差异基因表达，并利用生物信息学方法获得力信号转导调控网络，为全面理解种植体-骨组织界面之间的相互作用提供理论及应用基础。