胞质分裂中骨架蛋白对微管-皮层调控体系的生物力学作用研究

Animal cell cytokinesis is a mechanical deformation process characterized by a complex control system defined by interacting biochemical signals and mechanical modules. Microtubules (MTs) of the mitotic apparatus (MA) communicate with the cell cortex to stimulate cortical deformation of cytokinesis. MT plus end binding proteins （dynactin、EB1）and cytoskeleton （MTs， F-actin， Myosin II）were analyzed in this research, based on fluorescence live-cell imaging techniques, micropipette aspiration technique, local cytoskeletal inhibitors application technique, flourescence recovery after photobleaching (FRAP) et al., to investigate the mechanism of MTs biochemical signals direct the temporal and spatial control of the cortical distribution of F-actin, Myosin II and other mechanical elements during cortical deformation. Comparing the relation among different MTs-cortical deformation control system (passive cortical deformation, active cortical deformation and deformation with abnormal cytoskeleton). This study would give a better quantitative data to describe the relationship between MTs signals and cortical deformation. This research strategy might also serve as a useful tool and effective data for understanding the mechanism of cellular deformation.

动物细胞胞质分裂是由复杂的生化信号与力学元件操控的力学变形过程,细胞通过有丝分裂器微管对皮层的作用来调节胞质分裂皮层变形。本课题选取微管末端标记分子（dynactin和EB1），以及皮层骨架蛋白（肌动蛋白纤维，微管蛋白和肌球蛋白II）为研究对象，分析微管信号调节皮层中肌动蛋白纤维、肌球蛋白II等力学元件的作用规律。通过活细胞荧光蛋白分子示踪、外力诱导变形、细胞局部抑制剂处理、和荧光漂白恢复等技术研究胞质分裂中皮层变形的力学作用因子与微管调控信号在胞内相互作用的时效性和空间分布。对比该系统在主动变形、外源性力学刺激诱导变形和骨架蛋白异常状态下的响应。通过该项目，预期能够得到更好的定量数据用来描述微管信号与皮层变形之间的规律，并且为研究细胞变形提供有效的实验方法和数据支持。