Connexin43半通道/缝隙连接在康复训练改善创伤性脑损伤大鼠学习记忆能力中的作用的研究

Past study showed that rehabilitation training induced recovery of learning and memory after traumatic brain injury(TBI).In this study，we will investigate the mechanism for this recovery. Past studies demonstrated that Cx43 hemichannel/gap junction is involved in the regulation of learning and memory through gliotransmitters. Moreover,rehabilitation training can increase the expression of Cx43 protein.It suggested us that rehabilitation training induce recovery of the learning and memory after TBI through Cx43 hemichannel/gap junction. Accordingly, in present study, we are inclined to elucidate four questions by traumatic brain injury model:1.Is Cx43 hemichannel/gap junction involved in the regulation of learning and memory after TBI? 2.Does Cx43 hemichannel/gap junction regulate the learning and memory through gliotransmitters after TBI? 3.Does gliotransmitters act on the NMDA receptor after TBI? 4.Does rehabilitation training increase the learning and memory through Cx43 hemichannel/gap junction? In short, we will detect if there is the signal route for rehabilitation training→Cx43 hemichannel/gap junction→gliotransmitters→NMDA receptors→learning and memory recovery. Our study will provide the theoretical basis for the rehabilitative treatment of TBI.

本研究拟探讨康复训练改善创伤性脑损伤(TBI)大鼠学习记忆能力的分子机制。由于Cx43半通道/缝隙连接可以通过释放胶质递质调控学习记忆能力，而康复训练可以促进TBI大鼠Cx43蛋白的表达。这提示康复训练是否通过Cx43半通道/缝隙连接改善学习记忆能力？因此，本项目研究如下4个问题：1.TBI后，大鼠Cx43半通道/缝隙连接对学习记忆能力存在是否存在调控作用。2.TBI后，Cx43半通道/缝隙连接是否通过胶质递质参与对学习记忆调控。3.TBI后，Cx43半通道/缝隙连接释放的胶质递质是否最终作用于NMDA受体发挥调控作用。4. Cx43半通道/缝隙连接是否参与了康复训练促进创伤性脑损伤大鼠学习记忆能力恢复的调控。即探讨是否存在康复训练→Cx43 半通道/缝隙连接→胶质递质→NMDA受体→学习记忆能力恢复的信息传递途径。阐明上述问题，将为临床治疗创伤性脑损伤引起的高级神经功能障碍提供依据。

本研究拟探讨康复训练改善创伤性脑损伤(TBI)大鼠学习记忆能力的分子机制。由于Cx43 半通道/缝隙连接可以通过释放胶质递质调控学习记忆能力，而康复训练可以促进TBI 大鼠Cx43 蛋白的表达。这提示康复训练是否通过Cx43 半通道/缝隙连接改善学习记忆能力？因此，本项目发现：1.TBI 后，大鼠Cx43 半通道/缝隙连接对学习记忆能力存在调控作用。2.TBI 后，Cx43 半通道/缝隙连接通过胶质递质参与对学习记忆调控。3.TBI 后，NMDA 受体对学习记忆能力存在调控作用。4. Cx43 半通道/缝隙连接参与了康复训练促进创伤性脑损伤大鼠学习记忆能力恢复的调控，这将为临床治疗创伤性脑损伤引起的高级神经功能障碍提供依据。