P3HB4HB与GO/VRGO复合生物膜在牙槽窝骨缺损修复中的研究

The lack of alveolar bone caused by periodontitis, alveolar bone atrophy, trauma and tumor surgery usually leads to failure of implant surgery, which is a great challenge in dental clinical treatment. Guided bone regeneration (GBR) is the most commonly used technique to solve this problem. The key of GBR is the use of barrier membrane to separates bone defect area from surrounding soft tissue. At present, the most widely used barrier membrane in clinical application is animal-derived collagen membrane. But the poor mechanical property, fast degradation in vivo and lack of osteoinduction limit its development. In the previous study, VRGO has been successfully made from GO by high-pressure vapor reduction which is invented by our research team for the first time. And we have produced P3HB4HB/GO nanofibrous membrane by electrospinning, and demonstrated their promotion in adhesion, proliferation and osteogenic differentiation of stem cells. But the application of VRGO in bone tissue engineering is not studied. Therefore, this research, basing on previous study and focusing on shortcomings of collagen membrane, will design P3HB4HB/GO and P3HB4HB/VRGO membranes by electrospinning with ideal mechanical property, biocompatibility and osteogenic induction ability, and study their potential in bone defect repair of tooth socket, in order to lay the foundation for the researches and applications of the membrane in GBR technology.

牙周炎、牙槽骨萎缩、创伤及肿瘤手术等造成的种植区域的牙槽骨量不足，导致种植修复失败是当今口腔临床中亟待解决的问题。引导骨再生技术是解决此难题最常用的方法，其关键是使用屏障膜阻隔软组织与骨缺损区域。目前，临床应用最广泛的屏障膜是动物源性胶原膜，但其存在机械性能欠佳，体内降解速度过快且成骨诱导性不足等缺陷。课题组前期通过首创的高压蒸汽还原法将GO制备得到VRGO,并将P3HB4HB与GO复合制备为纳米纤维膜，初步证实其能够促进干细胞的黏附、增殖和成骨向分化，但对VRGO在骨组织工程中的应用未作研究。因此，本课题将结合既往研究基础，针对可吸收性胶原屏障膜的不足，拟以P3HB4HB为材料主体，通过静电纺丝技术构建含有GO或VRGO的生物膜，使其具有理想的机械性能和生物相容性，一定的骨引导和骨诱导能力，并研究其在牙槽窝骨缺损修复中的潜力，以期为该膜材料在口腔GBR技术的研究和应用打下基础。

牙周炎、牙槽骨萎缩、创伤及肿瘤手术等造成的种植区域的牙槽骨量不足，导致种植修复失败是当今口腔临床中亟待解决的问题。引导骨再生技术是解决此难题最常用的方法，其关键是使用屏障膜阻隔软组织与骨缺损区域。目前，临床应用最广泛的屏障膜是动物源性胶原膜，但其存在机械性能欠佳，体内降解速度过快且成骨诱导性不足等缺陷。本项目采用静电纺丝技术构建了含有P3HB4HB及GO或VRGO的电纺纤维支架。GO或VRGO的加入改善了P3HB4HB电纺支架的亲水性及理化性能，可以促进BMSCs的黏附、增殖和成骨分化。支架具有较好的生物相容性。在体内实验中，P3HB4HB电纺膜、P3HB4HB/GO电纺膜和P3HB4HB/VRGO电纺膜作为GBR膜均能起到良好的细胞屏障作用和支架作用，稳定和保护牙槽窝骨缺损区域的骨再生。P3HB4HB /VRGO电纺支架由于其优良的理化性能和生物安全性，在体内实验中表现出与Bio-Gide膜类似的结果。本项目为P3HB4HB /VRGO电纺膜在口腔GBR技术的研究和应用提供了有益的参考。