多模板分子印迹聚合物的新合成策略和印迹机理研究及应用

It is of great significance for food safety and environmental protection to develop an accurate, sensitive and efficient plant growth regulators (PGRs) multi residue separation and analysis method based on highly selective separation and enrichment. The objective of this study is to develop a reliable and efficient new strategy of synthesis of multi-template non-covalent molecularly imprinted polymers (MIPs). Molecular dynamics and quantum chemical methods are used to study the molecular imprinting mechanism and molecular recognition mechanism of multi-template MIPs at the molecular level. By combining multi-template imprinting with hollow sphere preparation technology, we attempt to synthesize multi-template plant growth regulators (PGRs) molecularly imprinted polymer hollow spheres (MIHSs) . The MIHSs thus have the advantages of group selectivity, template molecule easy to clean, high adsorption capacity and high mass transfer rate. The factors which have important effects on the size, dispersion and wall thickness of MIHSs are determined, and measure them quantitatively. Finally, the multi-template MIHSs will be used as SPE sorbent for the selective separation and enrichment of PGRs multi residues in agricultural products and environmental samples, combined with HPLC to achieve the purpose of accurate, sensitive and efficient detection of PGRs multi residues.

发展基于高选择性分离富集的样品前处理技术的准确、灵敏、高效的植物生长调节剂（PGRs）多残留分离分析方法，对食品安全和环境保护具有重要意义。本研究拟开发一种高效、可靠的合成非共价键多模板分子印迹聚合物的新策略，并在分子动力学模拟和量子化学计算方法的辅助下，从分子水平揭示多模板分子印迹聚合物的分子印迹机理和分子识别机制。并将多模板印迹与空心球制备技术结合，合成出对多种目标物具有高选择性、模板分子易洗脱干净、高吸附容量、高传质速率的植物生长调节剂分子印迹聚合物空心球（MIHSs）；确定影响MIHSs的尺寸、分散度及壁厚的因素及量化关系。最终，将多模板MIHSs作为SPE吸附剂，用于农产品和环境样品中PGRs多残留的高选择性分离富集，结合HPLC实现对PGRs多残留的准确、灵敏、高效检测。

发展基于高选择性分离富集的样品前处理技术的准确、灵敏、高效的植物生长调节剂（PGRs）多残留分离分析方法，对食品安全和环境保护具有重要意义。本研究发展了基于组选择性分子印迹材料分离富集结合HPLC检测的准确、灵敏、高效的PGRs多残留分离分析新方法。在分子动力学模拟及量子化学计算方法的辅助下，采用混合模板分子印迹结合膜技术，分别设计合成适用于有机相和水相的，同时对多种PGRs具有高选择性的分子印迹材料。该分子印迹材料内部同时具有识别多种PGRs的结合位点，并具有模板分子易洗脱干净、容量高、传质快的优点。以该材料为选择性吸附剂，选择性分离富集农产品和环境样品中多种PGRs，用HPLC进行精准检测。同时，本研究采用实验结合理论计算的方法从分子水平揭示混合模板分子印迹的机理。