硅基荧光磁性分子印迹材料选择性识别与分析菊酯类农药残留及其机理研究

By combining the advantages of surface imprinting technique,atom transfer radical polymerization (ATRP), multifunctional material toward separation and rhodamine,fluorescein and their derivatives, a new method will be obtained and used to selective recognition and determination of pyrethroid pesticides residues which based on the recognition effects of fluorescence probe and molecularly imprinted polymers (MIPs). In this work, the silicon-based fluorescent and magnetic MIPs will be designed and controlling synthesized. The target analyte will be recognized and determined synchronous by the fluorescence characteristics and specific adsorption of as-prepared composites. Then the as-prepared composites will be simply separated from the mother liquor due to the superparamagnetic matrix. By using the advanced characterization,the physical and chemical properties of as-prepared composites will be investigated, and the equilibrium，kinetics and thermodynamics laws will be discussed in detail. Finally, the method for the selective recognition and determination of pyrethroid pesticides will be obtained and applied to the fast and efficient determination of pyrethroid pesticides.

本课题拟集中表面分子印迹技术、ATRP活性可控聚合技术、磁性载体分离方法、罗丹明和荧光素类衍生物的优点，建立耦合荧光识别与分子印迹选择性识别的新方法，用于选择性识别与分析环境中菊酯类农药残留；设计、可控制备硅基荧光磁性分子印迹材料，利用硅基荧光磁性分子印迹材料基质的荧光特性和印迹聚合层的特异性吸附作用，实现对目标污染物分子的"绑定"和同步定量测定，利用基质的超顺磁性，实现在外磁场辅助下将饱和吸附的印迹材料与母液迅速分离，简化分离和再生程序；运用先进的表征手段，阐述硅基荧光磁性复合基质及其表面印迹材料的物理、化学特性，解释印迹聚合和选择性吸附过程中的平衡、动力学、热力学性质；建立选择性识别与分析菊酯类农药残留的方法，实现多种样品中痕量/超痕量菊酯类农药残留的快速和高效分析。

本课题集中表面分子印迹技术、ATRP 活性可控聚合技术、磁性载体分离方法、荧光素类衍生物的优点，建立荧光分子印迹选择性识别的新方法，用于选择性识别与分析环境中菊酯类农药残留；设计、可控制备硅基荧光磁性分子印迹材料，利用硅基荧光磁性分子印迹材料基质的荧光特性和印迹聚合层的特异性吸附作用，实现对目标污染物分子的"绑定"和同步定量测定，利用基质的超顺磁性，实现在外磁场辅助下将饱和吸附的印迹材料与母液迅速分离，简化分离和再生程序；实现多种样品中痕量/超痕量菊酯类农药残留的快速和高效分析。除了以磁性纳米材料作为印迹载体外，增加了硅基纳米材料作为印迹载体，扩展了印迹载体的种类。此外，在原有研究对象为菊酯类农药的基础上，我们增加了酚类物质，以提高项目成果的适用性。