花色苷调节微循环胰岛素转运早期防治糖尿病及其血管并发症作用研究

Anthocyanin, a water-soluble natural colorant, broadly exits in plants which has been a research focus for its health-promoting properties. Accumulating evidence suggests that anthocyanin could help prevent and treat diabetes. The underlying mechanisms remain unclear and warrant further study. Microvascular insulin resistance is the early event and play a key role in the development of whole body insulin resistant and diabetic vascular complications.Microvascular function influences the whole process of diabetes . It is recognized as a new target for the early prevention and treatment of diabetes and its complications. There is no report on the effect of anthocyanin on microvascular functions. In present study, we will focus on the microvascular insulin delivery function, an early and critical pathological change in insulin resistance and its vascular complications. We will establish endothelial cell and normal and high fat diet-fed animal models, using fluoresce and radioactive-labeled insulin as a tracing agent, applying insulin uptake and trans-endothelial transport transwell system in vitro, using insulin clamp and insulin delivery technique in vivo combined with molecular biology techniques to investigate the effect of anthocyanin on microvascular insulin delivery and its role in the development of insulin resistance. This study will further reveal the bioactivity of anthocyanin in body and provide a new theoretical basis and guidance for the anthocyanin interventions for the early prevention and treatment of diabetes and its vascular complications.

花青素是一种广泛存在于植物中的水溶性天然色素，因其具有促进健康的生物活性而备受关注。越来越多的证据显示花色苷类具有防治糖尿病的功效，但其机制亟待深入研究。微循环胰岛素抵抗不仅是全身胰岛素抵抗的始动因素和关键环节，而且在糖尿病血管并发症的发生起着重要作用，影响糖尿病的整个进程，是糖尿病及其血管并发症早期防治的新的靶点。花色苷对微循环功能的影响还未见研究。 本研究以微循环胰岛素转运功能这一胰岛素抵抗及糖尿病血管并发症早期关键的病理改变为切入点，拟采用正常和高脂细胞和动物模型，以荧光和放射性标记的胰岛素为示踪剂，在体外采用胰岛素摄取和胰岛素跨内皮转运系统，在体内结合胰岛素钳夹和胰岛素转运实验，并结合分子生物学方法从微循环胰岛素转运的角度研究花色苷对早期胰岛素抵抗的影响及其分子机制。为全面认识花色苷的生物活性，建立膳食花色苷早期防治糖尿病及其血管并发症提供新的理论基础和指导意义。