重组胃癌特异性血管生成抑制剂MG-Ad-IP10的研制

To improve the specificity of adenoviral gene therapy in vivo, a bacterial recombination system of adenovirus was used in the experimental therapy of gastric cancer. Two rounds of homologous recombination were carried out in E.coli strain BJ5183。A ScFv fragment of MG antibody was inserted into the fiber prtein of adenovirus, thus the resulting recombinant adenovirus was engineered to specifically infect MG positive gastric cancer cell. Due to the expression of angiostatic molecule, IP-10, in the genome of adenovirus, gastric cancer cells were efficiently inhibited. This experiment provides a preliminary data for the future clinical trial.

为增强重组腺病毒在体内治疗肿瘤的特异性，利用细菌重组系统，通过两次同源重组，将MG单链抗体基因融合表达于病毒颗粒表面的纤毛蛋白上，使重组腺病毒特异性感染MG抗原阳性胃癌细胞，以此为载体将血管抑制基因IP-10引入胃癌细胞并使之高效分泌表达，从≡裥云苹抵琢龈浇苣谄ぃ种莆赴┥ぃ蛑瘟朴τ糜诹俅蔡峁┯幸娴氖笛橐谰荨