表面抗氧化功能基团自组装的反应性及其生物应用研究(二)

This project is designed according to our previous original study in which the results showed that self-assembly of the surface-functionalized vitamin E could efficiently enhance free radical scavenging reactivity. As a result, we plan to design and synthesize a series of surface-functionalized antioxidants with different antioxidant groups, different nano-matrix structure or different types of conjunctions, and then to investigate the reaction kinetics between self-assembled antioxidant groups and DPPH free radical, to evaluate the π-π stacking interactions among antioxidant groups on the surface of self-assembled monolayer and the activation energies of the radical scavenging reaction. Subsequently, we hope to conclude why the rate constants for the free radical scavenging reaction of self-assembled antioxidants were significantly enhanced, which probably also provide a theoretical basis for the enhanced reactivities of other self-assembly of the surface-functionalized groups. Based on the above studies, we would further evaluate the kinetic protective effect of self-assembly of the surface-functionalized antioxidants on oxidative stress in vivo and in vitro and then disclose the pharmocolgical mechanism of the self-assembled antioxidants.

本项目基于申请人对表面自组装维生素E与自由基的高效动力学清除反应的原创性发现，拟定进一步通过设计与制备一系列不同抗氧化功能基团、不同纳米基底与不同链接方式的自组装抗氧化体系，深入探索所述自组装抗氧化体系与DPPH自由基的动力学反应规律与自组装方式对抗氧化功能基团间的π-π堆积相互作用和自由基清除反应活化能的调控规律。进而总结出表面抗氧化功能基团自组装致使反应速度增加的内在原因，并有望为其它类型表面功能化基团自组装体系的动力学效应提供一致的理论依据。在上述工作基础上，课题组进一步计划在细胞与活体生物两个层面内研究表面自组装抗氧化剂对氧化应激损伤过程的动力学保护效果，探索新型自组装抗氧化制剂的药理作用机制。

本课题基于申请人对表面自组装维生素E 与自由基的高效动力学清除反应的原创性发现，然后进一步通过设计与制备一系列不同抗氧化功能基团、不同纳米基底与不同链接方式的自组装抗氧化体系，深入探索所述自组装抗氧化体系与DPPH 自由基的动力学反应规律与自组装方式对抗氧化功能基团间的π-π堆积相互作用和自由基清除反应活化能的调控规律。进而总结出表面抗氧化功能基团自组装致使反应速度增加的内在原因是降低反应活化能，这为其它类型表面功能化基团自组装体系的动力学效应提供一致的理论依据。在上述工作基础上，课题组进一步在细胞与活体生物两个层面内研究表面自组装抗氧化剂对氧化应激损伤过程的动力学保护效果。此外，本课题还深入探索了新型自组装动力学原理在共价类药物设计领域的应用。