甘薯转录因子NAC083调控木质化影响储藏根发育的研究

Storage root is the important organ as energy storage in Sweetpotato, Lignification can affect the process of storage root development. However, there are very few studies the molecular mechanisms that transcription factor regulate lignification to affect the development of storage root. Compared initiation storage root with fibrous root transcription profiling data, the NAC family transcription factor NAC083 involved in lignification process to be find out. This project have already discovered interference with IbNAC083 can increase the lignification of storage root and affect the expansion of storage root. This find suggests IbNAC083 is an important negative regulated factor. Combined with detection the secondary cell wall structure and complement in Arabidopsis mutant vni2, this project will further testify IbNAC083 gene function by measurement starch and lignin content and change in hormone and phenotype in CRISPR/Cas9 knock out or overexpression IbNAC083 transgenic lines. Meanwhile, We expect to look for protein-protein interaction by yeast two-hybrid and biomolecular fluorescence complementation (BIFC) and so on. The downstream IbNAC083 regulation gene can be found by yeast one-hybrid. Combined the abiotic stress and hormone regulation, analysis molecular mechanism of NAC family regulate the thicken cell wall to affect the development of storage root. This study will provide the new methodology and techniques for improvement in crops yields.

储藏根是甘薯重要的储能器官，木质化影响其发育进程，因此内在转录因子调控木质化进而影响储藏根膨大的机制有待深入研究。前期分析甘薯起始膨大根与纤维根的转录谱差异，获得与木质化进程相关的NAC家族转录因子IbNAC083，发现干扰IbNAC083可以加剧储藏根的木质化，影响储藏根的膨大，表明IbNAC083是调控甘薯木质化的一个重要的负调控因子。本课题将进一步利用Crispr-Cas9编辑及过表达IbNAC083的转基因植株开展储藏根淀粉和木质素含量、激素及表型的变化，结合次生细胞壁结构的观察及拟南芥对应突变体互补实验来验证该基因的功能。同时利用酵母单双杂、BiFC等寻找与IbNAC083互作的蛋白及其下游调控基因，结合逆境胁迫和激素的影响，解析该NAC家族成员调控细胞壁加厚影响储藏根膨大的分子机制，为提高薯类作物的产量提供新的理论及技术。

储藏根是甘薯重要的储能器官，木质化影响其发育进程，因此内在转录因子调控木质化进而影响储藏根膨大的机制有待深入研究。前期分析甘薯起始膨大根与纤维根的转录谱差异，获得与木质化进程相关的NAC家族转录因子IbNAC083，发现干扰IbNAC083可以加剧储藏根的木质化，影响储藏根的膨大，实验表明IbNAC083是调控甘薯木质化的一个重要负调控因子。IbNAC083 RNAi植株的表型显示储藏根的膨大受到影响，影响到甘薯储藏根木质素的合成且淀粉的含量比野生型有所降低。同时利用酵母双杂、BiFC等寻找与IbNAC083互作的下游调控基因IbVND7蛋白是相互作用去负调控次生细胞壁的合成，结合逆境胁迫和激素的影响，解析该NAC家族成员调控细胞壁加厚影响储藏根膨大的分子机制，为提高薯类作物的产量提供新的理论及技术。