B.pseudomallei的fliC及其缺失段的重组表达、表位预测及应用

The infection of B.pseudomallei(Ara-) is common and dangerous in Hainan province. The present diagnosis with culture is low in positive rate amd take a long time. The treatment is delayed. The pathogenic B.pseudomallei (Ara-) strain from patients infected with B.pseudomallei and the nonpathogenic B.pseudomallei(Ara+) strain from isol are confused by the present diagnosis with culture. fliC is perticular for the B.pseudomallei strain, the missing unit of fliC is different between the pathogenic B.pseudomallei (Ara-) strain and the nonpathogenic B.pseudomallei(Ara+) strain. In our study, the fliC and the missing unit of fliC is got by PCR from the pathogenic B.pseudomallei (Ara- ) strain and the standard B.pseudomallei strain (K96243). The diagnosis by PCR for the B.pseudomallei (Ara-) strin is made. The recombinant of fliC or the missing unit of fliC , the forecasted epitope of fliC or the missing unit of fliC, or their antibody are used to determine the infection of B.pseudomallei(Ara-) by the method of ELISA with the serum or the secretion of the patients. The immunal response and the immunal protection of the recombinant FliC and missing unit of FliC are evaluated in the BALB/c mouse infected with B.pseudomallei strain (K96243),which lay the foundation of the vaccine and its antiserum.

类鼻疽伯克菌 B.pseudomallei(Ara-)感染是海南省常见、烈性传染病，目前细菌培养，阳性率低、耗时长、延误治疗，不能鉴别临床分离B.pseudomallei(Ara-)致病株、土壤分离B.pseudomallei(Ara+)非致病株。鞭毛C亚单位基因fliC是B.pseudomallei特有，致病株及非致病株的fliC缺失段不同。PCR扩增fliC、fliC缺失段, 建立快速、特异的PCR诊断方法；基因重组表达fliC、fliC缺失段获得rFliC、FliC缺失段，生物信息学预测其T、TH、B表位，合成多表位。rFliC、FliC缺失段、多表位或其抗血清分别与感染者血清或分泌物行ELISA，建立ELISA诊断方法。BALB小鼠B.pseudomallei(K96243)标准株感染模型,体内评价其免疫原性及免疫预防作用，为疫苗及抗血清研制打下基础。