氟酰胺对斑马鱼成鱼的毒性效应机制研究

As a succinate dehydrogenase inhibitors (SDHIs) fungicide, flutolanil has been widely used in the prevention and treatment of important diseases in rice fields throughout the world for many years. Unfortunately, previous studies found that flutolanil caused water pollution and induced toxicity on aquatic organisms. In the proposal, accumulation and residues of flutolanil in adult zebrafish and various organs as well as toxicity to zebrafish will be examined to identify the toxic target organs. Additionally, the association analysis of miRNA and mRNA combined with other various experiments will be carried out to determine the molecular biomarker and explore the toxic mechanisms of flutolanil in adult zebrafish. All these information are help to reveal the negative effects of flutolanil on aquatic ecosystem and gain better understanding of the toxic mechanism of flutolanil. The study would provide a theoretical basis for the rational use of flutolanil in the field as well as lay a theoretical foundation and technical support for the risk assessment of other SDHIs fungicides to aquatic organisms.

氟酰胺是一种高效的琥珀酸脱氢酶抑制剂（SDHIs）类杀菌剂，在世界各地的水稻纹枯病的防治中广泛使用，造成水环境污染，严重危害水生生物安全。本项目以斑马鱼成鱼为研究对象，通过成鱼发育毒性试验，以及氟酰胺在斑马鱼成鱼体内的蓄积和组织残留分布研究，结合组织病理技术，明确氟酰胺对斑马鱼成鱼的毒性效应和毒性靶器官；同时利用miRNA-mRNA联合分析技术，结合miRNA过量/沉默以及双荧光素酶报告基因手段，明确毒性分子标志物，进而揭示氟酰胺对斑马鱼成鱼的毒性机制。项目的开展有助于明确氟酰胺对水生生态环境的危害，为环境中氟酰胺的合理使用提供科学依据，为此类SDHI杀菌剂对水生生物的毒性评估提供借鉴。

氟酰胺是一种高效的琥珀酸脱氢酶抑制剂（SDHIs）类杀菌剂，在世界各地的水稻纹枯病 的防治中广泛使用，造成水环境污染，严重危害水生生物安全。本项目以斑马鱼成鱼为研究对 象，通过成鱼发育毒性试验，以及氟酰胺在斑马鱼成鱼体内的蓄积和组织残留分布研究，结合组织病理技术，明确氟酰胺对斑马鱼成鱼的毒性效应和毒性靶器官；同时利用miRNA-mRNA联合分析技术，明确毒性分子标志物，进而揭示氟酰胺对斑马鱼成鱼的毒性机制。结果显示氟酰胺在斑马鱼体内累积，存在富集效应，影响斑马鱼的生长发育，损伤斑马鱼肝脏，导致肝脏发生变性凋亡，组学结果显示其主要作用靶标关系对为mir26a-5p/socs1a以及mir29a/bicd2。项目的开展有助于明确氟酰胺对水生生态环境的危害，为环境中氟酰胺的合理使用提供科学依据，为此类SDHI杀菌剂对水生生物的毒性评估提供借鉴。