100Hz电针对帕金森病大鼠的治疗及机理研究

Parkinson's disease (PD) is a neurodegenerative disorder, mainly characterized by a massive and progressive degeneration of the dopaminergic neurons of the substantia nigra, resulting in a reduction of tyrosine hydroxylase (TH) and dopamine (DA). Up till now, there is still no method that can prevent or halting the progress of this disorder. Therefore, a comprehensive treatment strategy including the alternative therapy such as acupuncture and Chinese herbal medicine is still one of the clinical choices.In China, acupuncture has been reported effective in alleviating the symptoms of PD patients for decades. In addition, the employment of acupuncture in the treatment of other neurological disorders such as brain edema, spinal cord injury, the optic nerve atrophy, etc has also proved effective. However, the underlying biological mechanism supporting this traditional therapy still remains unknown. In the present study, we gave high and low frequency electro-acupuncture (EA) stimulation to the medial forebrain bundle (MFB) transected rats in order to find whether EA could attenuate the abnormal rotational behavior and dopaminergic neuron degeneration or increase the gene expression of GDNF and BDNF in the dopaminergic neurons..The main findings are: .1. Transaction of the medial forebrain bundle (MFB) with a wire knife result in abnormal ipsilateral rotational behavior of rats induced by amphetamine and reduction of DA in the striatum to about 50% two weeks after the transaction. 2. Both chronic low (2 Hz) and high (100Hz) frequency EA stimulation were effective in attenuating the rotational behavior within 2 weeks of MFB transaction. The same effect could last for at least 4 weeks in the animals stimulated with high frequency EA but not low frequency EA. 3. The content of DA in the striatum was increased significantly in the unlesioned side of both the low and high frequency EA stimulated rats. No increase had been found in the lesioned side. 4. High frequency EA could increase the gene expression of GDNF in the unlesioned side of substantia nigra reticular (SNr), and both side of the globus pallidus, while low frequency EA was effective in increasing gene expression of GDNF in the unlesioned side of globus pallidus. .5. High frequency EA could attenuate the degeneration of dopaminergic neurons in the SNc. The low frequency EA had no effect. 6. High frequency EA, but not the low frequency, could increase the gene expression of BDNF in the SNc and the ventral tegmental area (VTA) of the lesioned side of rats after MFB transaction, indicating that high frequency EA stimulation could be more effective than the low frequency EA in accelerating the synthesis of endogenous neurotrophic factors and in attenuating the degeneration of dopaminergic neurons. The present study suggests that adequate EA can attenuate the abnormal rotational behavior of MFB transected rat, prevent the degeneration of DA neurons and enhance the expression of BDNF mRNA in dopaminergic neurons and GDNF mRNA in global padalus..

依国际标准建立不同程度损伤的帕金森病（ＰＤ）大鼠模型，给予１００Ｈｚ的电针处理。观察电针对ＰＤ鼠异常行为的治疗作用；并采用神经化学、细胞形态学和分子生物学手段检测ＰＤ大鼠纹状体内多巴胺及其代谢物的含量、多巴胺Ｄ１和Ｄ２受体密度和基因表达、中脑腹侧部多巴胺转运体和某些神经营养因子的含量及基因表达等情况，以明确针刺防治帕金森病的机理。