自噬在UVB致角质形成细胞炎症因子分泌中作用及其分子机制

We demonstrated that UVB irradiation regulated differently autophagy of keratinocyte in a dose-dependent manner. Analysis of PCR array indicated that high dose of UVB irradiation can induce secretion of several inflammatory factors. It had been reported that UVB induced secretion of keratinocyte inflammatory factors, and autophagy negatively regulated secretion of IL-6 and TNF-αfrom keratinocyte. Thus, we plan to investigate the relationship between keratinocyte autophagy regulated by UVB and secretion of inflammatory factors. PCR array, antibodies array, and ELISA will be performed to assay the secretion of inflammatory factors from keratinocyte exposed different doses of UVB. Furthermore, the drugs incubation and RNAi will be utilized to regulate autophagy, accompanied with UVB irradiation, and then the secretion of inflammatory factors will be investigated. The chronic UVB skin damage mice model will be researched in vivo, and secretion of inflammatory factors will be assayed by PCR and antibodies array. Our project will be helpful to clarify above science question, and enrich the research of photobiology.

前期工作证实不同剂量UVB对体外培养角质形成细胞（KC）自噬具有差异调控效应，PCR array分析发现高剂量照射对数种炎症因子表达具有调控效应。相关研究表明UVB可以诱导KC分泌多种炎症因子，并且自噬负调节KC的IL-6和TNF-α分泌。因此，我们提出科学问题- - 差异调控的自噬在UVB致KC炎症相关细胞因子分泌中起到什么样的调节作用？本课题拟对体外培养的KC（原代细胞和细胞系），采用PCR array、细胞因子抗体芯片结合ELISA分析不同剂量UVB诱导出现的炎症相关细胞因子分泌水平，进而在照射前采用工具药或RNAi技术 调控自噬后分析上述炎症因子分泌水平的变化。初步分析自噬调控炎症因子分泌的分子机制。在体实验采用慢性UVB损伤小鼠模型，采用上述两种芯片结合组化标记分析炎症因子分泌水平差异。通过本课题可初步阐明上述科学问题，丰富以自噬为切入点的光生物学研究内容。

前期工作证实不同剂量UVB对体外培养角质形成细胞（KC）自噬具有差异调控效应，PCR array分析发现高剂量照射对数种炎症因子表达具有调控效应。相关研究表明UVB可以诱导KC分泌多种炎症因子，并且自噬负调节KC的IL-6和TNF-α分泌。因此，我们提出科学问题--差异调控的自噬在UVB致KC炎症相关细胞因子分泌中起到什么样的调节作用？本课题拟对体外培养的KC（原代细胞和细胞系），采用PCR array、细胞因子抗体芯片结合ELISA分析不同剂量UVB诱导出现的炎症相关细胞因子分泌水平，进而在照射前采用工具药或RNAi技术 调控自噬后分析上述炎症因子分泌水平的变化。初步分析自噬调控炎症因子分泌的分子机制。在体实验采用慢性UVB损伤小鼠模型采用上述两种芯片结合组化标记分析炎症因子分泌水平差异。通过本课题可初步阐明上述科学问题，丰富以自噬为切入点的光生物学研究内容。