胡杨应答盐胁迫转录组测序分析及基因表达调控网络的构建

Soil salinity is one of the most important factors to effect plant growth and development, decrease crop production, even endanger the ecological environment. Intensive study of salt tolerance mechanism of typical plant is very necessary to guide agricultural crops’ genetic improvement. As the only tree which can be formed to forest in arid saline areas in the northwest of China, Populus euphratica Oliv is a typical material for mechanism of salt and drought tolerance of woody plants. According to the past research projects at home and abroad of Populus euphratica physiological mechanism, Take the salt treatment and control of Populus euphratica cuttings of clones as material, determine the key period of sampling and the tissue site, sequencing on transcriptional level using RNA-seq and gene-chip technology, construction the salt stress Populus transcriptome library with the representative of time and space. Through the gene expression difference analysis of the different parts and different salt treatment, we have more in-depth understanding of characteristics of different parts of Populus euphratica in response to salt stress, find out the key gene and protein of Populus euphratica of salt tolerance. Using the bioinformatics technology and public database resources, construct the gene regulatory network of Populus euphratica responding to salt stress, get the sequence characteristics and the functions of genes related to resistance of Populus euphratica, dig out the excellent candidate gene resources, mining candidate gene resistant of Populus euphratica, provide gene resources for genetic improvement of Populus euphratica, provide important reference to analyze molecular interaction mechanism of salt tolerance for Populus euphratica.

土壤盐渍化是影响植物生长发育、降低作物产量、甚至危及生态环境的主要逆境因素之一。深入研究植物尤其是典型植物的耐盐机制，对指导农林作物的遗传改良具有重要意义。胡杨(Populus euphratica)是我国西北干旱盐碱地带唯一能形成森林的高大乔木树种，是进行木本植物耐盐研究的典型材料。项目根据国内外胡杨生理的研究基础，采用盐处理和对照胡杨扦插无性系为材料，确定取样的关键时期与组织部位，利用RNA-seq测序和基因芯片技术,构建具有时间和空间代表性的盐胁迫胡杨转录组文库。通过不同部位对不同盐处理时间点响应的基因表达情况的综合分析，比较深入理解胡杨不同部位对盐胁迫响应的特点，找出胡杨耐盐性的关键基因和蛋白。充分利用生物信息学技术和公共数据库资源，构建胡杨应答盐胁迫的基因表达调控网络，获得胡杨抗逆相关基因的序列特征和功能定位,为胡杨的遗传改良提供基因资源，为解析胡杨耐盐的分子机制提供重要参考。

土壤盐渍化是影响植物生长发育、降低作物产量、甚至危及生态环境的主要逆境因素之一。深入研究植物尤其是典型植物的耐盐机制，对指导农林作物的遗传改良具有重要意义。胡杨(Populus euphratica)是我国西北干旱盐碱地带唯一能形成森林的高大乔木树种，是进行木本植物耐盐研究的典型材料。本项目通过三年的工作，完成了胡杨无性系材料的盐胁迫实验和生理生化指标的测定，进行了盐胁迫条件下的转录组和miRNA测序工作，并对数据进行了一定的整理分析，包括基因表达、功能注释及参与的代谢途径等，鉴定到一批差异表达的基因。在此基础上，整合公共数据库资源，利用贝叶斯网络模型对有功能注释信息的基因构建了表达调控网络，获得胡杨抗逆相关基因的序列特征和功能定位,为胡杨的遗传改良提供基因资源，为解析胡杨耐盐的分子机制提供重要参考，为分子水平上研究多年生林木独特的解剖学、生理学及遗传学属性，以及林木植物生长发育规律提供新的思路和切入点。胡杨本身具有的抗旱抗盐能力,在治理西部的水土流失、改善西部生态环境中具有重要作用,因此对胡杨耐盐的深入研究更具有实际意义。