双标志物血检动脉粥样硬化微型电化学传感器的研究

Advanced oxidation protein products (AOPPs) are formed by reaction of plasma proteins with chlorinated oxidants, and have been considered as novel markers of oxidant-mediated protein damage. The variation of AOPPs content can lead to important changes of oxide low density lipoprotein (oxLDL) in atherosclerotic plaques. And increased levels of AOPP were described as an independent risk factor for atherosclerotic (AS). The research of AOPPs is still in primary stage. And the pathogenic mechanism involved remains unclear..Gold nanoparticles (AuNPs) based electrochemical biosensor for determination of AOPPs and low density lipoprotein cholesterol (LDL-C) will be designed in application of inkjet-printing technology. Modification of new AuNPs materials on microchannels could enhance electron transfer of ITO based electrodes as well as improve selectivity and sensitivity in bio-electrochemical systems. .In order to control the AOPPs concentration, small interfering RNA (siRNA) will be used as a tool for regulation of AOPPs expression. Antigen and antibody interaction depend on the new complex of siRNA-AuNPs-antiAOPPs. When AOPPs move through detection area, the coverage of AOPPs aptamars over the surface of the electrode will change the electron transfer between the electrode and a redox mediator upon binding of AOPPs. .The goal of this study is prove the AOPPs-dependent pathogenesis of atherosclerosis that affects the production of LDL-C. The fabricated electrochemical biosensors are able to determine AOPPs and LDL-C with high sensitivity, stability and selectivity. We hope the new bio-electrochemical systems act as a promising electrochemical platform for AS treatment.

动脉粥样硬化（AS）型心血管疾病的致病机理尚不明确，其独立危险因素晚期氧化蛋白产物（AOPPs）和低密度脂蛋白胆固醇（LDL-C）之间的相互关联，极少被关注。申请人拟设计制备AS双标志物的微型电化学传感器，通过对双标志物识别的精准操控，实时同步高效测定血浆中AOPPs、LDL-C的浓度，建立双物质量化的内在关联，探明AOPPs对AS发生发展的作用机理；探索动脉粥样硬化临床微量血检的快速、精准测试。本项目拟可控制备多体系金纳米颗粒，通过表面修饰调控电荷高效传导，达到为电极表面的电子传递提供快速通道的目的。同时，基于小干扰RNA（siRNA）抑制基因表达的高效性，解决传统药物引起的非特异性作用和偏靶效应。利用喷墨打印技术，设计开发单路进样、双通道分析的微传感器，以电化学方法实现对AOPPs和LDL-C浓度的同步快速、灵敏高效检测。

动脉粥样硬化（AS）型心血管疾病的致病机理尚不明确，其独立危险因素晚期氧化蛋白产物（AOPPs）和低密度脂蛋白胆固醇（LDL-C）之间的相互关联，极少被关注。申请人设计制备了AS双标志物的微型电化学传感器，通过对双标志物识别的精准操控，实时同步高效测定血浆中AOPPs、LDL-C的浓度，建立双物质量化的内在关联，探索AOPPs对AS发生发展的作用机理；探索动脉粥样硬化临床微量血检的快速、精准测试。本项目制备了可控多体系金纳米颗粒，通过表面修饰调控电荷高效传导，达到为电极表面的电子传递提供快速通道的目的。同时，基于小干扰RNA（siRNA）抑制基因表达的高效性，解决传统药物引起的非特异性作用和偏靶效应。利用喷墨打印技术，设计开发单路进样、双通道分析的微传感器，以电化学方法实现对AOPPs和LDL-C浓度的同步快速、灵敏高效检测。