黑灵芝多糖对丙烯酰胺致小肠细胞损伤的保护作用及其分子机制研究

How to use natural products to inhibit the toxicity of acrylamide (AA) is a research hotspot but also a difficulty in the field of environmental and food safety. Our previous researches have indicated that the polysaccharide from Ganoderma atrum (PSG-1) has such potential effects against AA-induced oxidative damage in intestinal epithelial cells. However, whether it affects the repair and regeneration ability of intestinal epithelium cells such as enzymes development, cytokine secretion and EGF / EGFR synthetic ability, and which upstream molecular signaling pathways is involved in such protection effects of PSG-1 are unclear. Therefore, in order to have a comprehensive understanding of the protective effects of PSG-1 against the AA-induced intestinal injury, its effects on the oxidation state, enzyme development, cytokine secretion, and proliferation/repair functions, cell permeability and tight junction protein expression of intestinal epithelial cells will be studied in vivo and in vitro. Its underlying mechanisms will also be discussed by investigating the MAPKS mediated apoptosis signaling pathway at the cellular level. This project will provide a scientific basis for early prevention of acrylamide toxicology. And also it will provide a theoretical guidance for the produce of high value-added food products from Ganoderma atrum polysaccharides.

如何利用天然产物抑制丙烯酰胺的毒性是目前环境和食品安全领域的研究热点和难点。我们前期研究表明黑灵芝多糖PSG-1具有潜在的预防和抑制丙烯酰胺(AA)引起的小肠细胞氧化损伤的作用，但是它是否影响肠上皮的生长和修复再生过程（如酶发育、细胞因子分泌、EGF/EGFR合成等），它又会通过何种种上游信号通路发挥作用等目前尚不清楚。因此本研究拟在细胞和动物水平上将PSG-1应用于AA诱导的肠道损伤的保护，从整体和细胞水平探讨PSG-1对小肠上皮细胞氧化还原、酶发育、细胞因子分泌、生长修复、细胞通透性和紧密连接蛋白表达等功能影响及其相互关系，期望全面找出PSG-1 对AA导致的小肠损伤的保护特点，并从MAPKS 介导的信号通路入手，在细胞水平上揭示PSG-1对AA诱导的小肠黏膜和上皮细胞毒性的预防、保护作用机制。本项目可为膳食预防丙烯酰胺中毒和黑灵芝多糖高附加值产品的生产提供科学依据和理论指导。

如何利用天然产物抑制丙烯酰胺的毒性是目前环境和食品安全领域的研究热点和难点。我们前期研究表明黑灵芝多糖PSG-1具有潜在的预防和抑制丙烯酰胺(AA)引起的小肠细胞氧 化损伤的作用，但是它是否影响肠上皮的生长和修复再生过程(如酶发育、细胞因子分泌 、EGF/EGFR合成等)，它又会通过何种种上游信号通路发挥作用等目前尚不清楚。因此本研究拟在细胞和动物水平上将PSG-1应用于AA诱导的肠道损伤的保护，从整体和细胞水平 探讨PSG-1对小肠上皮细胞氧化还原、酶发育、细胞因子分泌、生长修复、细胞通透性和紧密连接蛋白表达等功能影响及其相互关系，期望全面找出PSG-1 对AA导致的小肠损伤的保护特点，并从MAPKS 介导的信号通路入手，在细胞水平上揭示PSG-1对AA诱导的小肠黏 膜和上皮细胞毒性的预防、保护作用机制。本项目可为膳食预防丙烯酰胺中毒和黑灵芝多糖高附加值产品的生产提供科学依据和理论指导。