反义肝素酶cDNA、PI-88协同反义MMP9cDNA抗肺癌转移研究

Recently, studies showed that heparanase and matrix metalloproteinases ( MMPs ) could degrade heparan sulfate protoglycan ( HSPG ) and collagen of extracellular matrix specifically and respectively. Some cancer cells express heparanase and MMP9 at a high level, and these cancer cells become more aggressive and easier to metastasis. It suggested that inhibiting the expression of heparanase and MMP9 would inhibit the invasiveness and metastasis of cancer. We found that lung cancer expressed heparanase and MMP9 at a high level, and it closely correlated with the invasiveness and metastasis of lung cancer. Based on it, we plan to transfect antisense heparanase cDNA or antisense MMP9 cDNA or both, to inhibit their expression, so as to inhibit the infiltration and metastasis of lung cancer effectively. Now, our studies showed that transfection of antisense heparanase cDNA or antisense MMP9 cDNA separately could inhibit the invasiveness and metastasis potential of high-metastastic lung cancer cells in different extent. Transfection of both antisense cDNAs is under studying.

反义肝素酶cDNA、肝素酶抑制剂PI-88或它们协同反义MMP9cDNA转染或作用于人高转移性肺┫赴担魅匪嵌匀烁咦菩苑伟┫赴谔迥谕獾那窒妥颇芰Φ挠跋臁＝匆甯嗡孛竎DNA、PI-88或它们协同反义MMP9cDNA注入含高转移性肺癌的裸鼠体内，证明它们对裸鼠体内肺癌自发性转移的抑制作用。由此以期为临床抗肺癌转移的研究开辟一条新途径。