基于聚集诱导发光胶束的化学发光探针用于细胞内过氧化氢检测

Hydrogen peroxide is one of the most common reactive oxygen species in the organism. The changes of concentrations of hydrogen peroxide in vivo directly affect the physiological function of the organism. Highly sensitive and selective detection of hydrogen peroxide in vivo still faces great challenges. The aggregation-induced emission (AIE) probes are favored in the field of luminescence analysis due to their unique luminescence behaviors. Without exogenous excitation source, chemiluminescence analysis can effectively avoid the interference of background light and autofluorescence of biomolecules. The synthesis of analogs of amphiphilic surfactants with AIE characteristics will be achieved by attaching hydrophobic tails and a mitochondria-targeting cationic head into suitable AIE luminophores, and the formed nano-micelles will obtain high emission efficiency. The peroxalate compounds can be physically encapsulated into the hydrophobic core of such luminescent micelles. Taking the aggregated AIE luminophores in micelles as the energy acceptor, we can regulate the length of the hydrophobic tail to achieve efficient chemiluminescence resonance energy transfer. As a result, a mitochondria-targeting hydrogen peroxide probe with high sensitivity and good selectivity will be constructed and used for long-time tracking and imaging analysis of hydrogen peroxide in living cells. The success of this project will expand the cross-study of AIE and chemiluminescence field, and also is of great significance for the fundamental research and real applications of AIE and chemiluminescence.

过氧化氢作为生物体内最常见的活性氧分子，其浓度变化直接影响机体的生理机能。生物体内过氧化氢的高灵敏、专一性检测仍然面临巨大的挑战。聚集诱导发光（AIE）探针因其独特的发光行为，在发光分析领域备受青睐。化学发光分析无需外源性激发光源，可有效避免背景光和生物分子自体荧光的干扰。本项目拟以AIE发光团为母体，在其两端分别修饰疏水尾基和具有线粒体靶向性的阳离子头基，制备类表面活性剂的两亲型AIE分子，在水介质中经自组装获得高效发光纳米胶束。将过氧草酸酯类化合物经物理包裹进发光胶束的疏水内核，以胶束中聚集的AIE发光团为能量受体，通过调控疏水尾基的长度，实现高效的化学发光能量转移，构建灵敏度高、选择性好且具有线粒体靶向性的过氧化氢化学发光探针，并用于细胞内过氧化氢的长时间跟踪检测和成像分析。本项目的成功实施将拓展AIE与化学发光领域的交叉和延伸，对AIE与化学发光的基础与应用研究具有重要意义。

生物体内过氧化氢的高灵敏、专一性检测对研究其生理病理作用至关重要。本项目以聚集诱导发光（AIE）基团为母体，制备了一系列类表面活性剂的AIE两亲分子，在水介质中自组装获得高效发光纳米胶束。探索了其在水溶液中的自组装性能以及形成胶束的增溶机制，实现了胶束中增溶位置的精确测定；实验验证了化学发光共振能量转移（CRET）效率与供受体距离的定量关系，进而实现了发光胶束内CRET效率的可控调节，构建出高灵敏的过氧化氢化学发光胶束探针。本项目的成功实施拓展了AIE与化学发光的交叉研究，促进了聚集策略在化学发光分析中的延伸，对化学发光的基础与应用研究具有重要意义。