HIPEs模板构筑形状记忆多孔柔性印迹聚合物选择性分离纯化河豚毒素及机理研究

Investigation of novel methods for selective separation and purification of tetrodotoxin (TTX) , and improvement of TTX yield and purity from puffer fish organ extract, have been of extremely important significance in science, economy and society. In this project, the porous and flexible imprinted polymers with shape-memory performance (HFS-CMIPs) will be synthesized by Pickering high internal phase emulsions (HIPEs) templates and will be applied to selective separation and purification of TTX molecule. With water soluble phenyl boric acid monomer monomer and TTX as water phase, rubber modified carbon black composites as stable particles, and glycidyl methacrylate and photoinitiator as oil phase, they will be emulsified to form O/W type HIPEs. HFS-CMIPs will be prepared by low-temperature photoinitiating polymerization and ring-opening modification reaction. Advanced characterization methods will be used to test and explain the HFS-CMIPs’ porous micro structure, controllable regulation of flexibility and solvent-response shape memory. With the static separation experiments, it will explore the equilibrium, dynamic and thermodynamic behavior during the process of TTX adsorption and separation onto HFS-CMIPs, and investigate HFS-CMIPs recognition ability and mechanism of TTX. The novel method for selective separation and purification of TTX molecules from puffer fish viscera extract will be established, and TTX products with high yield and purity are expected to obtain.

探索河豚毒素(TTX)选择性分离纯化新方法，提高豚毒鱼类脏器萃取液中TTX得率和纯度，具有极其重要的科学、经济和社会意义。本课题拟利用Pickering高内相乳液(HIPEs)模板法构建形状记忆多孔柔性印迹聚合物(HFS-CMIPs)，并用于选择性分离纯化TTX。研究拟以水溶性苯硼酸单体、TTX等为水相，橡胶修饰的炭黑复合材料为稳定粒子，甲基丙烯酸缩水甘油酯和光引发剂为油相，乳化形成O/W型HIPEs；经低温光引发聚合和开环修饰反应制备HFS-CMIPs；运用先进表征手段测试并阐述HFS-CMIPs多孔微结构、柔性和溶剂响应形状记忆可控调节规律；结合静态分离实验，探索HFS-CMIPs吸附分离TTX过程中平衡、动力学和热力学行为，研究HFS-CMIPs对TTX识别能力与机制；建立选择性分离纯化豚毒鱼类脏器萃取液中TTX分子的新方法，获得高得率和纯度的TTX产品。

本课题将柔性聚合物基体材料（离子橡胶、纤维素、壳聚糖等）与微/纳材料（二氧化硅、氧化石墨烯、偕胺肟单体、冠醚功能化氧化石墨烯）、乳液聚合、分子印迹等相耦合，制备不同多孔柔性聚合物吸附材料，用于选择性分离富集河豚毒素、木犀草素等目标物；运用多种表征手段，阐释多孔柔性聚合物吸附材料的物理、化学特性；通过静态吸附试验，研究了多孔柔性吸附材料可控吸附分离河豚毒素等目标物的平衡、动力学和选择性识别行为，并构建相应的模型；利用乳液稳定机理将二氧化硅等纳米粒子用于无氟环保泡沫溶液性能优化、无卤阻燃剂制备及聚合物、生物质材料阻燃，无氟环保泡沫溶液在水成膜泡沫灭火剂方面具有良好的应用前景，无卤阻燃剂的应用使提高了相关高分子聚合物、生物质材料的使用安全性。通过本项目的顺利实施，促进了乳液聚合法制备柔性多孔材料方面的发展，为多孔柔性聚合物吸附材料及功能化微/纳材料的制备和应用提供新思路，拓展了相关材料的应用领域。