智能中空印迹微球选择性提取抗生素及分离行为和机理研究

By combining the advantages of microorganism,Pickering emulsion polymerization, molecularly imprinting technique and intelligent-responsive materials, smart (such as magnetic, thermoresponsive, and light responsive) and hollow imprinted microspheres will be synthesized with microorganism as stabilizers and applied to controlled and selective recognition，separation and enrichment of β-lactam antibiotics. With the help of binding sites and smart switches (magnet,temperature and light) of smart and hollow imprinted microspheres, the target β-lactam antibiotics will be selective recognition and enrichment, respectively. By using the advanced characterization,the physical and chemical properties of as-prepared smart and hollow imprinted microspheres will be investigated, and the equilibrium，kinetics and thermodynamics of Pickering imprinting polymerization，selective recognition and enrichment will be discussed in detail. Finally, the method for the controlled and selective recognition，separation and enrichment of β-lactam antibiotics will be obtained.

本课题拟集中菌类微生物、Pickering乳液聚合、分子印迹技术和智能响应性材料的优点，制备菌类微生物稳定化的智能响应（如磁敏型、温敏型、光敏型等）中空印迹微球，并用于可控选择性分离/提取β-内酰胺类抗生素；利用智能响应中空印迹微球的印迹位点实现对目标物的识别，利用磁场、温度和光源等响应"开关"实现对目标物的选择性分离提取；运用多种表征手段，阐释基于微生物菌体的磁敏型、温敏型、光敏型和多重响应型中空印迹微球的物理、化学特性，解释Pickering印迹聚合、可控选择性分离过程的平衡、动力学和热力学性质，研究识别与释放的机制；建立发酵液中β-内酰胺类抗生素可控识别/分离提取的新方法。

本课题将生物质材料（酵母细胞、壳聚糖颗粒、木质素等）与Pickering乳液聚合、分子印迹技术和智能响应性材料（磁性、温敏、光敏等材料）耦合，制备中空、多孔的智能响应印迹材料，并用于可控选择性分离富集β-内酰胺类抗生素；利用智能响应中空印迹材料的印迹位点实现对目标物的选择性识别，利用磁场、温度和光源等响应“开关”实现对目标物的选择性吸附分离；运用多种表征手段，阐释生物质材料稳定乳液聚合法制备的智能响应型中空印迹材料的物理、化学特性；通过静态吸附试验，研究了智能响应中空印迹材料可控选择性吸附分离β-内酰胺类抗生素的平衡、动力学和选择性识别行为，并构建相应的模型和分离富集方法；利用双乳液聚合制备酸碱双功能、多级孔结构的中空多孔催化材料，并用于催化纤维素制备5-羟甲基糠醛。运用多种方法对中空多孔催化材料进行形貌、结构、催化性能等表征和研究，通过两亲性纳米颗粒稳定油水体系，提高了多孔催化材料的热稳定、机械强度等材料性能。通过本项目的顺利实施，促进了生物质材料在乳液聚合制备新材料方面的发展，为智能响应分子印迹聚合物及多孔材料的制备和应用提供新思路，拓展了乳液聚合的应用领域。