谷胱甘肽S-转移酶基因多态性与机体氟水平对特发性男性不育易感性研究

Idiopathic male infertility is defined as the cause of the disease is still not found, the proportion of male infertility is 40~75%, and it is increasing year by year, in which the interaction of genetic polymorphisms and environmental factors may be one of the important pathogenic factors. Previous studies and our previous studies have indicated that the genetic polymorphisms of metabolic enzymes may play an important role in male infertility. Guizhou Province is one of the most serious pollution caused by combustion of coal type endemic fluorosis of China, the past and our previous study that coal-burning fluorosis through a variety of ways to affect male rat reproductive function and male reproductive endocrine changes. Based on the previous research, this project uses case control study, using high selective electrode method, PCR, RLFP-PCR, ELISA, TUNEL, flow cytometry and other experimental methods. To investigate the GSTM1, GSTT1 and GSTP1gene polymorphism in idiopathic male infertility plays an important role; And the relationship between serum, the body's fluorine levels in urine and semen is likely to through the effect on endocrine level, the level of oxidative stress pathways lead to the occurrence of male infertility. In order to understand the genetic polymorphism of GSTs gene and the level of fluoride in the body maybe is one of the factors that lead to the idiopathic male infertility. And to provide scientific basis for diagnosis and treatment of male infertility.

特发性男性不育症是指到目前仍未发现明确的致病原因，在男性不育症中所占比例为40~75%，且呈逐年上升趋势，其中基因遗传多态性与环境因素相互作用可能是其重要的致病因素之一。既往及我们前期研究表明代谢酶基因多态性可能在男性不育症中扮演重要的角色。贵州省是我国燃煤污染型氟中毒流行最严重省份之一，既往及我们前期研究认为燃煤型氟中毒可通过多种途径影响雄性大鼠的生殖功能和男性生殖内分泌的改变。该项目在前期的研究基础上采用病例-对照研究的方法，应用高选择性电极法、PCR、RLFP-PCR、ELISA、TUNEL、流式细胞仪等实验方法。探讨GSTM1、GSTT1和GSTP1基因多态性在特发性男性不育症中扮演的重要角色；血清、尿液及精液等机体氟水平是否可能通过影响机体内分泌水平、氧化应激水平等途径导致男性不育的发生；从而了解我省GSTs基因的遗传多态性和机体氟水平相互作用可能是导致特发性男性不育的因素之一。

特发性男性不育症是指到目前仍未发现明确的致病原因，在男性不育症中所占比例为40~75%，且呈逐年上升趋势，其中基因遗传多态性与环境因素相互作用可能是其重要的致病因素之一。既往及我们前期研究表明代谢酶基因多态性可能在男性不育症中扮演重要的角色。贵州省是我国燃煤污染型氟中毒流行最严重省份之一，既往及我们前期研究认为燃煤型氟中毒可通过多种途径影响雄性大鼠的生殖功能和男性生殖内分泌的改变。该项目在前期的研究基础上采用病例-对照研究的方法，应用高选择性电极法、PCR、RLFP-PCR、ELISA等分子生物学实验方法。经研究发现GSTM1、GSTT1和GSTP1等代谢酶基因亚型多态性在特发性男性不育症患者人群的频率分布以及和正常人群中基因多态性频率存在分布的差异，发现GSTs基因多态性对研究对象血清、尿液氟水平以及机体氧化应激水平有显著影响影响。因此，GSTs基因缺失或突变可能是增加燃煤型致机体高氟水平致男性不育发生的易感性因素之一。.