乳杆菌对Wnt/β-catenin信号转导通路因子的影响与绝经后宫颈病变相关性研究

Cervical cancer is a common malignant tumor, the peak of its incidence usually occurred on the age of respectively in reproductive (40 years old), Also, the age of postmenopausal (60 years old).The incidence of cervical cancer is directly related to HPV infection. For the postmenopausal age women, why it’s have the second peak, whether the pathogenesis for postmenopausal age is different from the reproductive age.There was few studies focus on this question, whether domestic or overseas. As a result, the question still didn’t solved. In the previously study, we found that the reproductive age infection was related to the microenvironment of cervix. So the question is: what’s induced the infection of the postmenopausal age? The reduction of vaginal lactobacillus in postmenopausal age will increased the susceptibility to HPV infection. Also, the Wnt/β - catenin signal pathway caused the cervical lesion. Therefore, this research intends to detect vagina lactobacillus and its capacity of producing hydrogen peroxide, also the test of HPV. The experiment will choose sample from the menopause women, reproductive women and patients with cervical lesions. In order to preliminary reveal the characteristics of vagina lactobacillus changes and HPV infection for postmenopausal women.The β- cantenin, E -cadherin, Vimentin, N-cadherin are important regulatory role in Wnt/β-catenin signaling pathway. We develop a test to detect the different cervical lesion between women in menopause and postmenopausal age. Furthermore, Lactobacillus fermentation in the different substrate and time will influence the expression approach for the cell Siha and Hela. So, the study wills analysis the correlation between lactobacillus inducing Wnt/β-catenin signaling pathway factors. And affection of cervical lesions in postmenopausal.

宫颈癌由HPV感染引起，其发病高峰分别在40岁和60岁左右。为何出现宫颈癌发病第二高峰，其发病机制是否有别于育龄期？本课题组前期研究表明育龄女性阴道乳杆菌与宫颈病变相关。绝经后女性宫颈病变发病机制是否因阴道乳杆菌减少，使HPV易感性增加从而对Wnt/β-catenin信号转导通路因子产生影响，导致宫颈病变发生。因此，本研究拟通过对绝经、未绝经健康女性及宫颈病变患者阴道乳杆菌及其产过氧化氢能力、HPV检测，初步明确绝经后女性阴道乳杆菌变化与HPV型别感染谱；通过对绝经、未绝经正常及宫颈病变组织Wnt/ β-catenin信号转导通路调节因子ß-cantenin、E-cadherin、Vimentin、N-cadherin检测，及不同浓度乳杆菌发酵滤液、不同时间作用下宫颈癌Siha、HeLa细胞系对上述因子表达，探讨乳杆菌对Wnt/β-catenin信号转导通路因子影响与绝经后宫颈病变相关性。

宫颈癌由HPV感染引起，其发病高峰分别在40岁和60岁左右。为何出现宫颈癌发病第二高峰，其发病机制是否有别于育龄期？本课题组前期研究表明育龄女性阴道乳杆菌与宫颈病变相关。绝经后女性宫颈病变发病机制是否因阴道乳杆菌减少，使HPV易感性增加从而对Wnt/β-catenin信号转导通路因子产生影响，导致宫颈病变发生。因此，本研究拟通过对绝经、未绝经健康女性及宫颈病变患者阴道乳杆菌、HPV检测，初步明确绝经后女性阴道乳杆菌变化与HPV型别感染谱；通过对绝经、未绝经正常及宫颈病变组织Wnt/ β-catenin信号转导通路调节因子ß-cantenin、E-cadherin、Vimentin、N-cadherin检测，及不同浓度乳杆菌发酵滤液、不同时间作用下宫颈癌Siha、HeLa细胞系对上述因子表达，探讨乳杆菌对Wnt/β-catenin信号转导通路因子影响与绝经后宫颈病变相关性。