五味子醇甲对老年痴呆证痴呆前期色氨酸代谢调节作用研究

Alzheimer's Disease（AD）is threatening life quality of the old population worldwide. The early prevetion of AD is supposed to be the optimal treatment for these irreversible neurodegenerations. In preliminary experiments, the abnormal metabolism of tryptophan was detected before AD occurred. And schizandrin, one of the main components in Shengmai-san, was found to be more relevant to these tryptophan metabolites. So the quantitative analysis of tryptophan metabolites, the morphological changes of astrocyte and the expression of Aβ and its related enzymes should be taken in the course of AD. The activities of related enzymes of tryptophan from small intestine, liver and brain tissue should be detected. Moreover, the AD rat model will be pre-treat with schizandrin, and its regulation on the tryptophan metabolites will be detected as a treatment mechanism research. These experiments would be helpful for early diagnosis of AD and taking preventions.

老年痴呆症（AD）是严重威胁全球老年人的生活质量的医学及社会学难题。该病为神经退行性疾病，具有不可逆性，早期干预成为控制AD的最佳途径。痴呆证前期本团队前期研究中发现AD发展各阶段的代谢特征及生物标记物，AD大鼠模型的色氨酸通路在痴呆前期表现出严重代谢异常。而生脉散对疾病的进程具有明显的延缓作用，并对色氨酸的代谢异常具有一定调节作用，而生脉散体内成分五味子醇甲与色氨酸通路的调节有较高关联性。因此本次实验在复制AD模型的过程中定向研究色氨酸代谢通路，重点关注色氨酸代谢产物的含量-时间变化、星形胶质细胞形态学变化以及Aβ和相关酶的表达。同时研究色氨酸代谢酶在小肠、肝脏和脑组织的表达。建立老年痴呆发生前色氨酸代谢与AD发生发展过程中的各现象间关系。在此基础上阐明五味子醇甲干预作用及其机制，揭示五味子醇甲调节的色氨酸通路靶点。为AD早期诊断和生脉散预防AD的药效物质基础研究奠定基础。