褐藻羊栖菜耐受低渗胁迫的代谢应答机制解析

In order to eliminate other wild algae, freshwater or lower gravities of seawater is usually employed to the brown alga Hizikia fusiformis (hijiki or hiziki) for a short period, indicating its tolerance to hypotonic stress. Osmotic stress is one of the major abiotic stresses for most algae. As most studies were concerning about the hypertonic stress, physiological mechanisms of tolerance to hypotonic stress of algae are not fully understood. This project focuses on responses of the brown alga hijiki to short-term (or acute) hypotonic stress, using comparative physiology approach combining methods of systems biology including proteomics and metabolomics. Then analyze the typical physiological characteristics related to osmotic stress and overall physiological metabolic networks to investigate the possible tolerance mechanism of hijiki to hypotonic stress at physiological level. As an important foundation work, analyzing the effect of hypotonic stress on the survival of hijiki and its mechanism is helpful to explore the tolerance of algae to abiotic stress. In another aspect, it will also provide the necessary theoretical basis for the “freshwater pesticide” technology.

羊栖菜育苗过程中常采用短时淡水浸泡方法防治杂藻侵害，显示出羊栖菜具有耐受低渗胁迫的特殊能力。渗透胁迫是大多数海藻面临的主要非生物胁迫，但目前研究主要关注高渗胁迫，对藻类耐受低渗胁迫的生理机制了解较少。本项目侧重于研究短期（急性）低渗胁迫下褐藻羊栖菜的应对机制，利用比较生理学方法结合系统生物学研究手段的蛋白质组学和代谢组学分析，通过对渗透胁迫相关的典型生理特性及整体生理代谢网络的系统分析，在生理水平上解析羊栖菜耐受低渗胁迫的机理，为进一步探讨深入的分子机制奠定基础。解析低渗胁迫对羊栖菜的胁迫作用及机理，是探讨藻类应对非生物胁迫的重要基础工作，也将为淡水除害技术提供必要的理论依据。

羊栖菜是我国沿海地区近岸养殖的具有重要经济价值的大型褐藻，为了保证种苗健康生长，其育苗过程常采用短时淡水浸泡方法防治杂藻侵害，显示出羊栖菜具有耐受低渗胁迫的特殊能力；渗透胁迫是大多数海藻面临的主要非生物胁迫，但以往研究主要关注高渗胁迫，对藻类耐受低渗胁迫的生理机制了解较少。本项目侧重研究了短期（急性）低渗胁迫下褐藻羊栖菜的应对机制，利用比较生理学方法结合系统生物学研究手段的蛋白质组学和代谢组学分析，通过对渗透胁迫相关的典型生理特性及整体生理代谢网络的系统分析，在生理水平上解析了羊栖菜耐受低渗胁迫的初步机理，为进一步探讨深入的分子机制奠定了基础。解析低渗胁迫对羊栖菜的胁迫作用及机理，是探讨藻类应对非生物胁迫的重要基础工作，也将为淡水除害技术提供了必要的理论依据。