重金属污染影响褐藻羊栖菜代谢的毒性机制解析

Heavy metal pollution in the coastal waters is of environmental concern. Algae are an important component of the marine ecosystem and occupy the base of the food chain. The consumption of heavy metal contaminated algae poses a threat to people's health. Investigation of heavy metal toxicity in algae and the mechanism is not only the basis to making explorations into the way of pollutants disturb marine ecosystem, but is essential theory for maintaining function and balancing of marine ecosystems. Reducing and avoiding their entry into the human body via food chain is based on learning the mechanism of heavy metal toxicity to algae..Hijiki (Hizikia fusiformis) is a type of macro algae that was cultured in coastal waters with great commercial value. Proteomics and metabolomics is applied to investigate the physiological responses of Hijiki exposed to high concentrations of the typical heavy metal pollutants (Cu, Zn, Cd, and Pb) for short time and lower concentrations during longer periods of time in this project. Bioinformatics analysis is conducted based on the analyzing the molecular variation between both Hijiki in laboratory exposures and with Hijiki exposed in the field. Systems biology focuses on complex interactions in biological systems which is complementary to the investigations of the traditional physiology analysis that limited in the number of biological indicators or phenotypes. Systems biology research would open new avenues for further studies on the mechanism of heavy metal toxicity in the algae because that can help us to recognize typical metabolites profiling and unique metabolic pathway in the algae exposed to different types of heavy metal pollutants.

重金属污染已经成为近岸海域环境的严重问题。藻类是海洋生态系统的重要组成部分，并有可能通过食物链向人类传递重金属污染的危害。研究近岸海域重金属污染对藻类的毒害作用及其机理，既是探讨环境污染对近岸海洋生态系统扰动机制、维护海洋生态系统功能和生态平衡的重要基础工作，也是减轻或避免重金属污染通过食物链危害人类的前提和保障。.羊栖菜是近岸养殖、具有重要经济价值的大型褐藻，本项目应用蛋白质组学和代谢组学技术研究羊栖菜生理代谢对重金属（铜、锌、镉、铅）高浓度急性暴露和低浓度长期暴露的响应过程，并利用生物信息学手段与污染海域羊栖菜的代谢响应在分子水平上进行联配与比较。这些系统生物学的研究方法可以弥补以往研究中仅"局部"观察少数生物学指标或生理学现象的不足，同时有利于甄别藻类与不同重金属污染物毒性相关的典型代谢特征和特异代谢途径，增加了深入认知重金属污染对藻类毒性机理的可能性。

重金属污染已经成为近岸海域环境的严重问题，研究近岸海域重金属污染对藻类的毒害作用及其机理，既是探讨环境污染对近岸海洋生态系统扰动机制、维护海洋生态系统功能和生态平衡的重要基础工作，也是减轻或避免重金属污染通过食物链危害人类的前提和保障。羊栖菜是近岸养殖、具有重要经济价值的大型褐藻，本项目应用蛋白质组学和代谢组学技术研究了羊栖菜生理代谢对重金属（铜、锌、镉、铅）高浓度急性暴露和低浓度长期暴露的响应过程，明确了羊栖菜在蛋白质水平和代谢物水平上响应几种重金属污染的分子基础，总结了羊栖菜响应几种重金属污染的生理代谢途径和代谢特征，解析了几种重金属污染影响羊栖菜代谢的毒性机制。其中，我们有关铜、镉影响羊栖菜代谢的系统生物学分析结果在大型海藻胁迫生物学研究领域具有一定的特色，得到了同行学者的关注、引用、借鉴和评述。