急性粒细胞白血病HRX/EEN转基因小鼠模型的建立

To study the biological function of fusion gene HRX-EEN and its role in leukemogenesis, thus provide an ideal animal model for anti-leukemia drug screening. Method We constructed HRX-EEN fusion gene from three different DNA fragments and inserted it into hCG transgenic vector. G0 transgenic mice were obtained by microinjection of the recombined DNA into the pronucleus of zygotes, followed by implantation of the injected zygotes into pseudopregnant mice. We tested the ：integration of the transgene by PCR and its expression by RT-PCR. Results The sequence of recombined HRX-EEN gene is confirmed by sequencing. PCR testing revealed a total of 7 G0 transgenic mice, which were then mated with C57 wild type mice. Except for mouse No. 35 which died, the other ones all had their F1 offsprings. From these 6 lines of transgenic mice, HRX-EEN gene was found to be stably expressed in 3 lines by RT-PCR. Up to now, all transgenic mice expressing the fusion gene have no obvious abnormal phenotypes. Conclusion A transgenic mice model in which the HRX-EEN fusion gene can be stably expressed has been established.

急性粒细胞白血病(AML)染色体易位t(11,19)(q23,p13)导致融合基因HRX-EEN形成，该融合蛉?.5kb，与白血病的发病密切有关，拟用转基因技术建立白血病HRX-EEN转基因小鼠Ｐ停芯考毙粤Ｏ赴籽〉姆⒉』恚秆⌒碌驴怪琢鲆┪锾峁├硐氲亩锬Ｐ汀?..