运用基因锁技术建立HCV 结构基因的转基因小鼠模型

Hepatitis C virus (HCV) structured gene transgenic mouse model, in.which HCV gene spatiotemporally expresses, is generated using Tet-on gene.regulation system. One advantage of this system, which is extremely useful for.examining immunological reactions against transgene products such as infectious.agents, is that the transgenic animal is immunocompetent for the transgene.product. This model allows HCV gene to express specifically in the liver after.birth so as to overcome immune tolerance produced during embryonic period and.immune escape against hepatitis virus antigen. The model will approach more.natural conditions in vivo after virus infection expresses antigen, supply ideally.experimental system to expound mechanisms of HCV immunological reactions.and pathogenesis, develop a disease model and new method for Hepatitis C.control and drug screening.

为克服HCV研究尚无经济可行的动物模型和敏感细胞培养系统，为在个体水平上探讨HCV结构基因的生物学功能在致病中的作用。本课题将运用四环素调控系统基因锁技术建立HCV结构虻淖蛐∈竽Ｐ筒⑻教制渲虏』啤４四Ｐ湍苋肏CV结构基因在小鼠出生后在其肝脏中表达，克服免疫耐受障碍，更接近与病毒感染体内自然情况，为丙肝发病机制的研究以及防治开辟一条新路