猴头菇多糖对MDRV致番鸭肠黏膜免疫功能损伤的修复及其机制

Our previous research found that infection of muscovy duck reovirus(MDRV) mainly caused damage to intestinal mucosal tissue and intestinal mucosal immune system, thus resulting in intestinal mucosal immune function down-regulated even lost and nutrient malabsorption, and fatality rate was up to 60%-80%. Since there was no effective drugs for prevention and treatment up to now, this disease was still bringing about enormous loss to the southern waterfowl industry. When developing drugs for prevention and treatment, we found that the Hericium erinaceus polysaccharide (HEPS) could effectively control the infection of MDRV, facilitate recovery of MDRV-infected muscovy ducks and reduce mortality, while the mechanism of action was still unknown. Thereby, this study focused on the repair of HEPS on MDRV-induced intestinal mucosal injury and intestinal mucosal immune suppression in muscovy ducks, studied its effects on the morphology of the intestinal mucosa; analyzed the amount of cells in association with intestinal mucosal immunity, secretion production of sIgA, expression level of mucosal vascular addressin, content of immune function related cytokines, expression level of IL-2 and IL-6 mRNA in the intestinal tissue and so on, thus elucidating the mechanism of action of HEPS on the repair of intestinal mucosa, restore and enhance of mucosal immunity in MDRV-infected muscovy ducks, and providing a scientific basis for control of this disease.

项目组前期研究发现番鸭呼肠孤病毒（MDRV）感染番鸭主要引起肠道黏膜组织和肠道黏膜免疫系统损伤，造成肠道黏膜免疫功能低下甚至丧失和营养吸收障碍，病死率高达60%-80%。由于尚无有效防治药物，至今此病仍然给南方水禽业带来巨大损失。我们研发此病防治药物时，发现猴头菇多糖（HEPS）能有效地防治番鸭呼肠孤病毒（MDRV）感染，促进MDRV感染番鸭康复，降低死亡率，但对其作用机制尚不清楚。为此，本项目以HEPS对MDRV感染致雏番鸭肠黏膜损伤和肠道黏膜免疫抑制的修复作用为切入点，研究其对肠黏膜形态结构的影响；分析肠道黏膜免疫相关的细胞数量、sIgA分泌量、黏膜血管地址素表达水平、免疫机能相关细胞因子的含量、肠道组织中IL-2和IL-6mRNA表达水平等，进而阐明HEPS对感染MDRV番鸭肠黏膜免疫功能损伤的修复及其作用机制，为该病防治提供科学依据。

番鸭呼肠孤病毒（MDRV）感染雏番鸭主要引起肠道黏膜组织和肠道黏膜免疫系统损伤，造成肠道黏膜免疫功能低下甚至丧失和营养吸收障碍，病死率高达60%-80%，给南方水禽业带来巨大损失。我们研究发现，猴头菇多糖（HEPS）能有效地防治MDRV感染，促进MDRV感染番鸭康复，降低死亡率，但对其作用机制尚不清楚。本项目主要针对HEPs在MDRV感染致雏番鸭肠黏膜损伤和肠道黏膜免疫抑制的修复过程的作用机制进行了研究。本项目组已完成项目计划书所有研究内容，研究结果如下：.（1）项目组建立了MDRV感染的疾病模型；.（2）确定了HEPs饮水防治MDRV感染最适浓度；.（3）检测分析了HEPs对试验番鸭肠道MDRV复制水平的影响。.（4）研究分析了HEPs对试验番鸭肠道黏膜形态结构、肠道黏膜免疫相关的细胞数量、黏膜sIgA分泌量、黏膜血管地址素表达水平及肠道黏膜抗氧化能力的影响；.（5）检测分析了HEPs对试验番鸭肠道黏膜免疫机能相关细胞因子的含量及肠道组织中IL-2和IL-6 mRNA表达水平的影响；.（6）课题组在完成了所有项目计划书任务目标的基础上，还完成了测序分析试验番鸭肠道组织转录组的差异、检测分析HEPs对MDRV感染番鸭淋巴细胞归巢相关因子的影响和HEPs对MDRV感染RAW264.7细胞TLR3信号转导通路的影响等三方面新增研究内容。.本研究结果明晰了MDRV感染导致的肠道黏膜损伤，引起感染番鸭肠道黏膜免疫抑制和营养吸收障碍的病理过程及其机制，丰富发展了MDRV感染引起免疫抑制的分子机制；明确了HEPS饮水预防用药对防治番鸭呼肠孤病毒感染有确切效果，阐明HEPs对MDRV感染番鸭肠黏膜的修复和保护作用，揭示了HEPs对MDRV感染致免疫抑制番鸭的肠道黏膜修复及免疫调节作用机制，为HEPs防治MDRV感染提供了科学途径和依据。.项目实施期间发表研究结果相关论文11篇，其中SCI期刊论文 3篇，权威期刊论文5篇、核心期刊论文 3篇；培养硕士研究生 4名。