NF-Kappa B信号通路对Nacrein启动子的转录活性及蛋白表达和分泌的调控作用研究
基本信息
结项摘要
基质蛋白在珍珠形成过程中发挥了重要的调控作用，研究NF-Kappa B信号通路对基质蛋白基因的转录及表达调控作用，不仅有助于揭示珍珠形成的分子机制，而且有助于了解贝类的天然免疫系统与珍珠形成的内在联系。本课题以合浦珠母贝珍珠基质蛋白Nacrein为研究对象，利用软件预测和EMSA实验证实该蛋白基因启动子中可能存在的NF-KappaB结合位点。运用定点突变技术和荧光素酶报告基因载体（pGL3）系统，研究NF-KappaB信号通路对Nacrein启动子转录活性的调控作用。用TNF-α和PDTC处理原代培养的外套膜细胞，应用Real-Time PCR检测培养细胞中IKK，Rel,Nacrein等基因的表达量的变化，用Western Blot、ELISA和金标免疫电镜技术等方法研究NF-KappaB信号通路对Nacrein蛋白表达和分泌的调控作用。为优良珍珠的培育及新型生物材料的研制提供理论依据。
项目摘要
项目成果
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数据更新时间：2023-05-31
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